Assembly of voltage-gated potassium channels: Conserved hydrophilic motifs determine subfamily-specific interactions between the α-subunits

Jia Xu, Weifeng Yu, Yuh Nung Jan, Lily Yeh Jan, Min Li

Research output: Contribution to journalArticle

Abstract

Voltage-gated potassium (K+) channels are assembled by four identical or homologous α-subunits to form a tetrameric complex with a central conduction pore for potassium ions. Most of the cloned genes for the α-subunits are classified into four subfamilies: Kv1 (Shaker), Kv2 (Shab), Kv3 (Shaw), and Kv4 (Shal). Subfamily-specific assembly of heteromeric K+ channel complexes has been observed in vitro and in vivo, which contributes to the diversity of K+ currents. However, the molecular codes that mediate the subfamily-specific association remain unknown. To understand the molecular basis of the subfamily-specific assembly, we tested the protein-protein interactions of different regions of α-subunits. We report here that the cytoplasmic NH2-terminal domains of Kv1, Kv2, Kv3, and Kv4 subfamilies each associate to form homomultimers. Using the yeast two-hybrid system and eight K+ channel genes, two genes (one isolated from rat and one from Drosophila) from each subfamily, we demonstrated that the associations to form heteromultimers by the NH2-terminal domains are strictly subfamily-specific. These subfamily-specific associations suggest a molecular basis for the selective formation of heteromultimeric channels in vivo.

Original languageEnglish (US)
Pages (from-to)24761-24768
Number of pages8
JournalJournal of Biological Chemistry
Volume270
Issue number42
StatePublished - Oct 20 1995

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Voltage-Gated Potassium Channels
Genes
Potassium
Two-Hybrid System Techniques
Hybrid systems
Yeast
Drosophila
Rats
Proteins
Ions
Electric potential

ASJC Scopus subject areas

  • Biochemistry

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Assembly of voltage-gated potassium channels : Conserved hydrophilic motifs determine subfamily-specific interactions between the α-subunits. / Xu, Jia; Yu, Weifeng; Jan, Yuh Nung; Jan, Lily Yeh; Li, Min.

In: Journal of Biological Chemistry, Vol. 270, No. 42, 20.10.1995, p. 24761-24768.

Research output: Contribution to journalArticle

Xu, Jia ; Yu, Weifeng ; Jan, Yuh Nung ; Jan, Lily Yeh ; Li, Min. / Assembly of voltage-gated potassium channels : Conserved hydrophilic motifs determine subfamily-specific interactions between the α-subunits. In: Journal of Biological Chemistry. 1995 ; Vol. 270, No. 42. pp. 24761-24768.
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