TY - JOUR
T1 - Assembly of Na,K-ATPase α-subunit isoforms with Na,K-ATPase β-subunit isoforms and H,K-ATPase β-subunit
AU - Lemas, M. Victor
AU - Yu, Hui Ying
AU - Takeyasu, Kunio
AU - Kone, Bruce
AU - Fambrough, Douglas M.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1994/7/15
Y1 - 1994/7/15
N2 - cDNA encoding an epitope tag was joined to cDNAs encoding the chicken Na,K-ATPase β1 and β2 and H,K-ATPase β-subunits to allow recognition of these β-subunits with the same monoclonal antibody during assembly assays. cDNAs encoding chicken Na,K-ATPase α1, α2, or α3 and Na,K-ATPase β1 or β2 or H,K-ATPase β-subunits were transiently coexpressed in mammalian cells. Subunit assembly was assayed by immune precipitation of α-isoforms with a monoclonal antibody to the epitope-tagged β-subunits. Each of the chicken α-isoforms assembled with each of the Na,K-ATPase β-subunits and the H,K-ATPase β-subunit. Each of the epitope-tagged β-subunits also assembled with a Na,K-ATPase/Ca-ATPase chimera that retained only 26 amino acids of the Na,K-ATPase α-subunit, demonstrating that all three β-subunits recognize this same α-subunit assembly site.
AB - cDNA encoding an epitope tag was joined to cDNAs encoding the chicken Na,K-ATPase β1 and β2 and H,K-ATPase β-subunits to allow recognition of these β-subunits with the same monoclonal antibody during assembly assays. cDNAs encoding chicken Na,K-ATPase α1, α2, or α3 and Na,K-ATPase β1 or β2 or H,K-ATPase β-subunits were transiently coexpressed in mammalian cells. Subunit assembly was assayed by immune precipitation of α-isoforms with a monoclonal antibody to the epitope-tagged β-subunits. Each of the chicken α-isoforms assembled with each of the Na,K-ATPase β-subunits and the H,K-ATPase β-subunit. Each of the epitope-tagged β-subunits also assembled with a Na,K-ATPase/Ca-ATPase chimera that retained only 26 amino acids of the Na,K-ATPase α-subunit, demonstrating that all three β-subunits recognize this same α-subunit assembly site.
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M3 - Article
C2 - 7518440
AN - SCOPUS:0028352750
SN - 0021-9258
VL - 269
SP - 18651
EP - 18655
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 28
ER -