Assembly and use of a broadly applicable neural cDNA microarray

Tanya Barrett, Chris Cheadle, William B. Wood, Diane Teichberg, David M. Donovan, William J. Freed, Kevin G. Becker, Marquis P. Vawter

Research output: Contribution to journalArticle

Abstract

cDNA microarrays provide an efficient method to analyze gene expression patterns in thousands of genes in parallel. In some cases, large unfocused collections of cDNAs have been used in hybridization studies, in others small logically defined collections of tissue specific arrays have been used. Here we describe the bioinformatic selection of 1152 named human cDNAs specifically designed for neuroscience applications, arrayed on nylon membranes at high density. cDNAs were chosen which represent all the major cellular types of the brain including; neurons, astrocytes, microglia, and oligodendrocytes. Gene families chosen include cell type specific markers, ion-channels, transporters, receptors, and cell adhesion molecules among many others. These arrays were used with region specific samples from human brain to determine mRNA expression profiles for each region. Used with 33-P labeled complex probes, this is a low cost, highly sensitive approach for the investigator to focus on tissue specific genes of interest where samples of limiting amounts of RNA are used. This selected set of brain-relevant cDNAs should be widely useful in the analysis of gene expression patterns from brain tissues as well as neural cell lines.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalRestorative Neurology and Neuroscience
Volume18
Issue number2-3
StatePublished - 2001
Externally publishedYes

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Oligonucleotide Array Sequence Analysis
Complementary DNA
Brain
Gene Expression Profiling
Genes
Nylons
Oligodendroglia
Cell Adhesion Molecules
Microglia
Neurosciences
Computational Biology
Ion Channels
Astrocytes
Research Personnel
RNA
Neurons
Costs and Cost Analysis
Cell Line
Messenger RNA
Membranes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neuropsychology and Physiological Psychology

Cite this

Barrett, T., Cheadle, C., Wood, W. B., Teichberg, D., Donovan, D. M., Freed, W. J., ... Vawter, M. P. (2001). Assembly and use of a broadly applicable neural cDNA microarray. Restorative Neurology and Neuroscience, 18(2-3), 127-135.

Assembly and use of a broadly applicable neural cDNA microarray. / Barrett, Tanya; Cheadle, Chris; Wood, William B.; Teichberg, Diane; Donovan, David M.; Freed, William J.; Becker, Kevin G.; Vawter, Marquis P.

In: Restorative Neurology and Neuroscience, Vol. 18, No. 2-3, 2001, p. 127-135.

Research output: Contribution to journalArticle

Barrett, T, Cheadle, C, Wood, WB, Teichberg, D, Donovan, DM, Freed, WJ, Becker, KG & Vawter, MP 2001, 'Assembly and use of a broadly applicable neural cDNA microarray', Restorative Neurology and Neuroscience, vol. 18, no. 2-3, pp. 127-135.
Barrett T, Cheadle C, Wood WB, Teichberg D, Donovan DM, Freed WJ et al. Assembly and use of a broadly applicable neural cDNA microarray. Restorative Neurology and Neuroscience. 2001;18(2-3):127-135.
Barrett, Tanya ; Cheadle, Chris ; Wood, William B. ; Teichberg, Diane ; Donovan, David M. ; Freed, William J. ; Becker, Kevin G. ; Vawter, Marquis P. / Assembly and use of a broadly applicable neural cDNA microarray. In: Restorative Neurology and Neuroscience. 2001 ; Vol. 18, No. 2-3. pp. 127-135.
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