Abstract
cDNA microarrays provide an efficient method to analyze gene expression patterns in thousands of genes in parallel. In some cases, large unfocused collections of cDNAs have been used in hybridization studies, in others small logically defined collections of tissue specific arrays have been used. Here we describe the bioinformatic selection of 1152 named human cDNAs specifically designed for neuroscience applications, arrayed on nylon membranes at high density. cDNAs were chosen which represent all the major cellular types of the brain including; neurons, astrocytes, microglia, and oligodendrocytes. Gene families chosen include cell type specific markers, ion-channels, transporters, receptors, and cell adhesion molecules among many others. These arrays were used with region specific samples from human brain to determine mRNA expression profiles for each region. Used with 33-P labeled complex probes, this is a low cost, highly sensitive approach for the investigator to focus on tissue specific genes of interest where samples of limiting amounts of RNA are used. This selected set of brain-relevant cDNAs should be widely useful in the analysis of gene expression patterns from brain tissues as well as neural cell lines.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 127-135 |
| Number of pages | 9 |
| Journal | Restorative Neurology and Neuroscience |
| Volume | 18 |
| Issue number | 2-3 |
| State | Published - 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
- Neuropsychology and Physiological Psychology
Cite this
Assembly and use of a broadly applicable neural cDNA microarray. / Barrett, Tanya; Cheadle, Chris; Wood, William B.; Teichberg, Diane; Donovan, David M.; Freed, William J.; Becker, Kevin G.; Vawter, Marquis P.
In: Restorative Neurology and Neuroscience, Vol. 18, No. 2-3, 2001, p. 127-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Assembly and use of a broadly applicable neural cDNA microarray
AU - Barrett, Tanya
AU - Cheadle, Chris
AU - Wood, William B.
AU - Teichberg, Diane
AU - Donovan, David M.
AU - Freed, William J.
AU - Becker, Kevin G.
AU - Vawter, Marquis P.
PY - 2001
Y1 - 2001
N2 - cDNA microarrays provide an efficient method to analyze gene expression patterns in thousands of genes in parallel. In some cases, large unfocused collections of cDNAs have been used in hybridization studies, in others small logically defined collections of tissue specific arrays have been used. Here we describe the bioinformatic selection of 1152 named human cDNAs specifically designed for neuroscience applications, arrayed on nylon membranes at high density. cDNAs were chosen which represent all the major cellular types of the brain including; neurons, astrocytes, microglia, and oligodendrocytes. Gene families chosen include cell type specific markers, ion-channels, transporters, receptors, and cell adhesion molecules among many others. These arrays were used with region specific samples from human brain to determine mRNA expression profiles for each region. Used with 33-P labeled complex probes, this is a low cost, highly sensitive approach for the investigator to focus on tissue specific genes of interest where samples of limiting amounts of RNA are used. This selected set of brain-relevant cDNAs should be widely useful in the analysis of gene expression patterns from brain tissues as well as neural cell lines.
AB - cDNA microarrays provide an efficient method to analyze gene expression patterns in thousands of genes in parallel. In some cases, large unfocused collections of cDNAs have been used in hybridization studies, in others small logically defined collections of tissue specific arrays have been used. Here we describe the bioinformatic selection of 1152 named human cDNAs specifically designed for neuroscience applications, arrayed on nylon membranes at high density. cDNAs were chosen which represent all the major cellular types of the brain including; neurons, astrocytes, microglia, and oligodendrocytes. Gene families chosen include cell type specific markers, ion-channels, transporters, receptors, and cell adhesion molecules among many others. These arrays were used with region specific samples from human brain to determine mRNA expression profiles for each region. Used with 33-P labeled complex probes, this is a low cost, highly sensitive approach for the investigator to focus on tissue specific genes of interest where samples of limiting amounts of RNA are used. This selected set of brain-relevant cDNAs should be widely useful in the analysis of gene expression patterns from brain tissues as well as neural cell lines.
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M3 - Article
C2 - 11847435
AN - SCOPUS:0034771420
VL - 18
SP - 127
EP - 135
JO - Restorative Neurology and Neuroscience
JF - Restorative Neurology and Neuroscience
SN - 0922-6028
IS - 2-3
ER -