Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites

Mei Zhao, Daisuke Sano, Curtis R. Pickering, Samar A. Jasser, Ying C. Henderson, Gary L. Clayman, Erich M. Sturgis, Thomas J. Ow, Reuben Lotan, Thomas E. Carey, Peter G. Sacks, Jennifer R. Grandis, David Sidransky, Nils Erik Heldin, Jeffrey N. Myers

Research output: Contribution to journalArticle

Abstract

Purpose: Human cell lines are useful for studying cancer biology and preclinically modeling cancer therapy, but can be misidentified and cross-contamination is unfortunately common. The purpose of this study was to develop a panel of validated head and neck cell lines representing the spectrum of tissue sites and histologies that could be used for studying the molecular, genetic, and phenotypic diversity of head and neck cancer. Methods: A panel of 122 clinically and phenotypically diverse head and neck cell lines from head and neck squamous cell carcinoma, thyroid cancer, cutaneous squamous cell carcinoma, adenoid cystic carcinoma, oral leukoplakia, immortalized primary keratinocytes, and normal epithelium was assembled from the collections of several individuals and institutions. Authenticity was verified by carrying out short tandem repeat analysis. Human papillomavirus (HPV) status and cell morphology were also determined. Results: Eighty-five of the 122 cell lines had unique genetic profiles. HPV-16 DNA was detected in 2 cell lines. These 85 cell lines included cell lines from the major head and neck primary tumor sites, and close examination shows a wide range of in vitro phenotypes. Conclusions: This panel of 85 genomically validated head and neck cell lines represents a valuable resource for the head and neck cancer research community that can help advance understanding of the disease by providing a standard reference for cell lines that can be used for biological as well as preclinical studies.

Original languageEnglish (US)
Pages (from-to)7248-7264
Number of pages17
JournalClinical Cancer Research
Volume17
Issue number23
DOIs
StatePublished - Dec 1 2011

Fingerprint

Neck
Head
Cell Line
Neoplasms
Head and Neck Neoplasms
Oral Leukoplakia
Adenoid Cystic Carcinoma
Human papillomavirus 16
Skin Neoplasms
Keratinocytes
Thyroid Neoplasms
Microsatellite Repeats
Molecular Biology
Squamous Cell Carcinoma
Histology
Epithelium
Phenotype
DNA
Research

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Zhao, M., Sano, D., Pickering, C. R., Jasser, S. A., Henderson, Y. C., Clayman, G. L., ... Myers, J. N. (2011). Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites. Clinical Cancer Research, 17(23), 7248-7264. https://doi.org/10.1158/1078-0432.CCR-11-0690

Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites. / Zhao, Mei; Sano, Daisuke; Pickering, Curtis R.; Jasser, Samar A.; Henderson, Ying C.; Clayman, Gary L.; Sturgis, Erich M.; Ow, Thomas J.; Lotan, Reuben; Carey, Thomas E.; Sacks, Peter G.; Grandis, Jennifer R.; Sidransky, David; Heldin, Nils Erik; Myers, Jeffrey N.

In: Clinical Cancer Research, Vol. 17, No. 23, 01.12.2011, p. 7248-7264.

Research output: Contribution to journalArticle

Zhao, M, Sano, D, Pickering, CR, Jasser, SA, Henderson, YC, Clayman, GL, Sturgis, EM, Ow, TJ, Lotan, R, Carey, TE, Sacks, PG, Grandis, JR, Sidransky, D, Heldin, NE & Myers, JN 2011, 'Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites', Clinical Cancer Research, vol. 17, no. 23, pp. 7248-7264. https://doi.org/10.1158/1078-0432.CCR-11-0690
Zhao, Mei ; Sano, Daisuke ; Pickering, Curtis R. ; Jasser, Samar A. ; Henderson, Ying C. ; Clayman, Gary L. ; Sturgis, Erich M. ; Ow, Thomas J. ; Lotan, Reuben ; Carey, Thomas E. ; Sacks, Peter G. ; Grandis, Jennifer R. ; Sidransky, David ; Heldin, Nils Erik ; Myers, Jeffrey N. / Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites. In: Clinical Cancer Research. 2011 ; Vol. 17, No. 23. pp. 7248-7264.
@article{7ca8553d78084f39ba2cfda8860a1fb6,
title = "Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites",
abstract = "Purpose: Human cell lines are useful for studying cancer biology and preclinically modeling cancer therapy, but can be misidentified and cross-contamination is unfortunately common. The purpose of this study was to develop a panel of validated head and neck cell lines representing the spectrum of tissue sites and histologies that could be used for studying the molecular, genetic, and phenotypic diversity of head and neck cancer. Methods: A panel of 122 clinically and phenotypically diverse head and neck cell lines from head and neck squamous cell carcinoma, thyroid cancer, cutaneous squamous cell carcinoma, adenoid cystic carcinoma, oral leukoplakia, immortalized primary keratinocytes, and normal epithelium was assembled from the collections of several individuals and institutions. Authenticity was verified by carrying out short tandem repeat analysis. Human papillomavirus (HPV) status and cell morphology were also determined. Results: Eighty-five of the 122 cell lines had unique genetic profiles. HPV-16 DNA was detected in 2 cell lines. These 85 cell lines included cell lines from the major head and neck primary tumor sites, and close examination shows a wide range of in vitro phenotypes. Conclusions: This panel of 85 genomically validated head and neck cell lines represents a valuable resource for the head and neck cancer research community that can help advance understanding of the disease by providing a standard reference for cell lines that can be used for biological as well as preclinical studies.",
author = "Mei Zhao and Daisuke Sano and Pickering, {Curtis R.} and Jasser, {Samar A.} and Henderson, {Ying C.} and Clayman, {Gary L.} and Sturgis, {Erich M.} and Ow, {Thomas J.} and Reuben Lotan and Carey, {Thomas E.} and Sacks, {Peter G.} and Grandis, {Jennifer R.} and David Sidransky and Heldin, {Nils Erik} and Myers, {Jeffrey N.}",
year = "2011",
month = "12",
day = "1",
doi = "10.1158/1078-0432.CCR-11-0690",
language = "English (US)",
volume = "17",
pages = "7248--7264",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "23",

}

TY - JOUR

T1 - Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites

AU - Zhao, Mei

AU - Sano, Daisuke

AU - Pickering, Curtis R.

AU - Jasser, Samar A.

AU - Henderson, Ying C.

AU - Clayman, Gary L.

AU - Sturgis, Erich M.

AU - Ow, Thomas J.

AU - Lotan, Reuben

AU - Carey, Thomas E.

AU - Sacks, Peter G.

AU - Grandis, Jennifer R.

AU - Sidransky, David

AU - Heldin, Nils Erik

AU - Myers, Jeffrey N.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Purpose: Human cell lines are useful for studying cancer biology and preclinically modeling cancer therapy, but can be misidentified and cross-contamination is unfortunately common. The purpose of this study was to develop a panel of validated head and neck cell lines representing the spectrum of tissue sites and histologies that could be used for studying the molecular, genetic, and phenotypic diversity of head and neck cancer. Methods: A panel of 122 clinically and phenotypically diverse head and neck cell lines from head and neck squamous cell carcinoma, thyroid cancer, cutaneous squamous cell carcinoma, adenoid cystic carcinoma, oral leukoplakia, immortalized primary keratinocytes, and normal epithelium was assembled from the collections of several individuals and institutions. Authenticity was verified by carrying out short tandem repeat analysis. Human papillomavirus (HPV) status and cell morphology were also determined. Results: Eighty-five of the 122 cell lines had unique genetic profiles. HPV-16 DNA was detected in 2 cell lines. These 85 cell lines included cell lines from the major head and neck primary tumor sites, and close examination shows a wide range of in vitro phenotypes. Conclusions: This panel of 85 genomically validated head and neck cell lines represents a valuable resource for the head and neck cancer research community that can help advance understanding of the disease by providing a standard reference for cell lines that can be used for biological as well as preclinical studies.

AB - Purpose: Human cell lines are useful for studying cancer biology and preclinically modeling cancer therapy, but can be misidentified and cross-contamination is unfortunately common. The purpose of this study was to develop a panel of validated head and neck cell lines representing the spectrum of tissue sites and histologies that could be used for studying the molecular, genetic, and phenotypic diversity of head and neck cancer. Methods: A panel of 122 clinically and phenotypically diverse head and neck cell lines from head and neck squamous cell carcinoma, thyroid cancer, cutaneous squamous cell carcinoma, adenoid cystic carcinoma, oral leukoplakia, immortalized primary keratinocytes, and normal epithelium was assembled from the collections of several individuals and institutions. Authenticity was verified by carrying out short tandem repeat analysis. Human papillomavirus (HPV) status and cell morphology were also determined. Results: Eighty-five of the 122 cell lines had unique genetic profiles. HPV-16 DNA was detected in 2 cell lines. These 85 cell lines included cell lines from the major head and neck primary tumor sites, and close examination shows a wide range of in vitro phenotypes. Conclusions: This panel of 85 genomically validated head and neck cell lines represents a valuable resource for the head and neck cancer research community that can help advance understanding of the disease by providing a standard reference for cell lines that can be used for biological as well as preclinical studies.

UR - http://www.scopus.com/inward/record.url?scp=80455136301&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80455136301&partnerID=8YFLogxK

U2 - 10.1158/1078-0432.CCR-11-0690

DO - 10.1158/1078-0432.CCR-11-0690

M3 - Article

VL - 17

SP - 7248

EP - 7264

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 23

ER -