Assay of endogenous opiate receptor ligands in human CSF and plasma

D. Naber; D. Pickar; R. A. Dionne; D. L. Bowie; B. A. Ewels; T. W. Moody; M. G. Soble; C. B. Pert

Assay of endogenous opiate receptor ligands in human CSF and plasma

D. Naber, D. Pickar, R. A. Dionne, D. L. Bowie, B. A. Ewels, T. W. Moody, M. G. Soble, C. B. Pert

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid and reliable radioreceptor assay for measuring endogenous opiate peptides in human CSF and plasma has been developed. The method is based on the competition between [ ³H]-[D-Ala ²]enkephalin-(L-leu-amide) ⁵ and biologically active opioids for binding sites using crude rat brain membranes. Sensitivity is about 250 fmol/ml sample; 100 μl of plasma or CSF inhibit the total binding within the range of 25-75%. At therapeutic concentrations, psychotropic drugs do not interfere with the assay. Elution profiles of CSF and plasma show two major peaks, probably representing endorphin-like and enkephalin-like compounds. Normal levels, expressed in β-endorphin-like activity, are 1.4-4.8 pmol/ml for extracted plasma and 7.1-15.8 pmol/ml for CSF.

Original language	English (US)
Pages (from-to)	83-91
Number of pages	9
Journal	Substance and Alcohol Actions/Misuse
Volume	1
Issue number	1
State	Published - 1980
Externally published	Yes

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Assay of endogenous opiate receptor ligands in human CSF and plasma",

abstract = "A rapid and reliable radioreceptor assay for measuring endogenous opiate peptides in human CSF and plasma has been developed. The method is based on the competition between [ 3H]-[D-Ala 2]enkephalin-(L-leu-amide) 5 and biologically active opioids for binding sites using crude rat brain membranes. Sensitivity is about 250 fmol/ml sample; 100 μl of plasma or CSF inhibit the total binding within the range of 25-75%. At therapeutic concentrations, psychotropic drugs do not interfere with the assay. Elution profiles of CSF and plasma show two major peaks, probably representing endorphin-like and enkephalin-like compounds. Normal levels, expressed in β-endorphin-like activity, are 1.4-4.8 pmol/ml for extracted plasma and 7.1-15.8 pmol/ml for CSF.",

author = "D. Naber and D. Pickar and Dionne, {R. A.} and Bowie, {D. L.} and Ewels, {B. A.} and Moody, {T. W.} and Soble, {M. G.} and Pert, {C. B.}",

year = "1980",

language = "English (US)",

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T1 - Assay of endogenous opiate receptor ligands in human CSF and plasma

AU - Naber, D.

AU - Pickar, D.

AU - Dionne, R. A.

AU - Bowie, D. L.

AU - Ewels, B. A.

AU - Moody, T. W.

AU - Soble, M. G.

AU - Pert, C. B.

PY - 1980

Y1 - 1980

N2 - A rapid and reliable radioreceptor assay for measuring endogenous opiate peptides in human CSF and plasma has been developed. The method is based on the competition between [ 3H]-[D-Ala 2]enkephalin-(L-leu-amide) 5 and biologically active opioids for binding sites using crude rat brain membranes. Sensitivity is about 250 fmol/ml sample; 100 μl of plasma or CSF inhibit the total binding within the range of 25-75%. At therapeutic concentrations, psychotropic drugs do not interfere with the assay. Elution profiles of CSF and plasma show two major peaks, probably representing endorphin-like and enkephalin-like compounds. Normal levels, expressed in β-endorphin-like activity, are 1.4-4.8 pmol/ml for extracted plasma and 7.1-15.8 pmol/ml for CSF.

AB - A rapid and reliable radioreceptor assay for measuring endogenous opiate peptides in human CSF and plasma has been developed. The method is based on the competition between [ 3H]-[D-Ala 2]enkephalin-(L-leu-amide) 5 and biologically active opioids for binding sites using crude rat brain membranes. Sensitivity is about 250 fmol/ml sample; 100 μl of plasma or CSF inhibit the total binding within the range of 25-75%. At therapeutic concentrations, psychotropic drugs do not interfere with the assay. Elution profiles of CSF and plasma show two major peaks, probably representing endorphin-like and enkephalin-like compounds. Normal levels, expressed in β-endorphin-like activity, are 1.4-4.8 pmol/ml for extracted plasma and 7.1-15.8 pmol/ml for CSF.

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Assay of endogenous opiate receptor ligands in human CSF and plasma

Abstract

ASJC Scopus subject areas

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