Assay development for detection and quantitation of fatty acid acyl conjugates of hydrocortisone by gas chromatography-electron capture negative ion mass spectrometry (GC-ECNI-MS)

Walter C. Hubbard, Carol Bickel, Robert P. Schleimer

Research output: Contribution to conferencePaper

Abstract

The assay development for detection and quantitation of fatty acid acyl conjugates of hydrocortisone (HC) is discussed. The gas chromatography-electron capture negative ion mass spectrometry (GC-ECNI-MS) techniques are used for the purpose. Fatty acid acylation of HC would alter the partitioning properties of the glucocorticoid making it more lipid-soluble and more likely to be cell-associated. Deacylation of the fatty acid conjugates of HC would also provide a mechanism through which the biological effect of the endogenous steroid may be prolonged. The sequence of fatty acid acylation and deacylation of HC in vivo may represent a mechanism through which HC modulates inflammation.

Original languageEnglish (US)
Pages481-482
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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    Hubbard, W. C., Bickel, C., & Schleimer, R. P. (2002). Assay development for detection and quantitation of fatty acid acyl conjugates of hydrocortisone by gas chromatography-electron capture negative ion mass spectrometry (GC-ECNI-MS). 481-482. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.