Aspergillus section Fumigati typing by PCR-restriction fragment polymorphism

Janet F. Staab, S. Arunmozhi Balajee, Kieren Marr

Research output: Contribution to journalArticle

Abstract

Recent studies have shown that there are multiple clinically important members of the Aspergillus section Fumigati that are difficult to distinguish on the basis of morphological features (e.g., Aspergillus fumigatus, A. lentulus, and Neosartorya udagawae). Identification of these organisms may be clinically important, as some species vary in their susceptibilities to antifungal agents. In a prior study, we utilized multilocus sequence typing to describe A. lentulus as a species distinct from A. fumigatus. The sequence data show that the gene encoding β-tubulin, benA, has high interspecies variability at intronic regions but is conserved among isolates of the same species. These data were used to develop a PCR-restriction fragment length polymorphism (PCR-RFLP) method that rapidly and accurately distinguishes A. fumigatus, A. lentulus, and N. udagawae, three major species within the section Fumigati that have previously been implicated in disease. Digestion of the benA amplicon with BccI generated unique banding patterns; the results were validated by screening a collection of clinical strains and by in silico analysis of the benA sequences of Aspergillus spp. deposited in the GenBank database. PCR-RFLP of benA is a simple method for the identification of clinically important, similar morphotypes of Aspergillus spp. within the section Fumigati.

Original languageEnglish (US)
Pages (from-to)2079-2083
Number of pages5
JournalJournal of Clinical Microbiology
Volume47
Issue number7
DOIs
StatePublished - Jul 2009

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Aspergillus fumigatus
Aspergillus
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Neosartorya
Multilocus Sequence Typing
Antifungal Agents
Nucleic Acid Databases
Tubulin
Computer Simulation
Sequence Analysis
Digestion
Databases
Genes

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Aspergillus section Fumigati typing by PCR-restriction fragment polymorphism. / Staab, Janet F.; Balajee, S. Arunmozhi; Marr, Kieren.

In: Journal of Clinical Microbiology, Vol. 47, No. 7, 07.2009, p. 2079-2083.

Research output: Contribution to journalArticle

Staab, Janet F. ; Balajee, S. Arunmozhi ; Marr, Kieren. / Aspergillus section Fumigati typing by PCR-restriction fragment polymorphism. In: Journal of Clinical Microbiology. 2009 ; Vol. 47, No. 7. pp. 2079-2083.
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