Artificial reporter gene providing MRI contrast based on proton exchange

Assaf A. Gilad, Michael T Mcmahon, Piotr Walczak, Paul T Winnard, Venu Raman, Hanneke W M Van Laarhoven, Cynthia M. Skoglund, Jeff W Bulte, Peter C Van Zijl

Research output: Contribution to journalArticle

Abstract

Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.

Original languageEnglish (US)
Pages (from-to)217-219
Number of pages3
JournalNature Biotechnology
Volume25
Issue number2
DOIs
StatePublished - Feb 2007

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ASJC Scopus subject areas

  • Microbiology

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