TY - JOUR
T1 - Artificial reporter gene providing MRI contrast based on proton exchange
AU - Gilad, Assaf A.
AU - McMahon, Michael T.
AU - Walczak, Piotr
AU - Winnard, Paul T.
AU - Raman, Venu
AU - Van Laarhoven, Hanneke W.M.
AU - Skoglund, Cynthia M.
AU - Bulte, Jeff W.M.
AU - Van Zijl, Peter C.M.
N1 - Funding Information:
We thank D.A. Kedziorek, V.P. Chacko and J. Zhang for experimental assistance. Supported by National Institutes of Health grants CA103175, NS045062, EB005252 and K01 EB006394.
PY - 2007/2
Y1 - 2007/2
N2 - Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.
AB - Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.
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U2 - 10.1038/nbt1277
DO - 10.1038/nbt1277
M3 - Article
C2 - 17259977
AN - SCOPUS:33846853038
SN - 1087-0156
VL - 25
SP - 217
EP - 219
JO - Nature biotechnology
JF - Nature biotechnology
IS - 2
ER -