Arterial levels of oxygen stimulate intimal hyperplasia in human saphenous veins via a ROS-dependent mechanism

Binata Joddar, Michael S. Firstenberg, Rashmeet K. Reen, Saradhadevi Varadharaj, Mahmood Khan, Rachel C. Childers, Jay L. Zweier, Keith J. Gooch

Research output: Contribution to journalArticle

Abstract

Saphenous veins used as arterial grafts are exposed to arterial levels of oxygen partial pressure (pO2), which are much greater than what they experience in their native environment. The object of this study is to determine the impact of exposing human saphenous veins to arterial pO2. Saphenous veins and left internal mammary arteries from consenting patients undergoing coronary artery bypass grafting were cultured ex vivo for 2 weeks in the presence of arterial or venous pO2 using an established organ culture model. Saphenous veins cultured with arterial pO2 developed intimal hyperplasia as evidenced by 2.8-fold greater intimal area and 5.8-fold increase in cell proliferation compared to those freshly isolated. Saphenous veins cultured at venous pO2 or internal mammary arteries cultured at arterial pO2 did not develop intimal hyperplasia. Intimal hyperplasia was accompanied by two markers of elevated reactive oxygen species (ROS): increased dihydroethidium associated fluorescence (4-fold, p2 is suggested by the observation that chronic exposure to tiron, a ROS scavenger, during the two-week culture period, blocked intimal hyperplasia. Electron paramagnetic resonance based oximetry revealed that the pO2 in the wall of the vessel tracked that of the atmosphere with a ∼30 mmHg offset, thus the cells in the vessel wall were directly exposed to variations in pO2. Monolayer cultures of smooth muscle cells isolated from saphenous veins exhibited increased proliferation when exposed to arterial pO2 relative to those cultured at venous pO2. This increased proliferation was blocked by tiron. Taken together, these data suggest that exposure of human SV to arterial pO2 stimulates IH via a ROS-dependent pathway.

Original languageEnglish (US)
Article numbere0120301
JournalPLoS One
Volume10
Issue number3
DOIs
StatePublished - Mar 23 2015
Externally publishedYes

Fingerprint

saphenous vein
Tunica Intima
Saphenous Vein
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt
hyperplasia
Hyperplasia
reactive oxygen species
Reactive Oxygen Species
Oxygen
oxygen
Mammary Arteries
Cell proliferation
Cell culture
Grafts
Partial pressure
arteries
breasts
Paramagnetic resonance
Muscle
Monolayers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Joddar, B., Firstenberg, M. S., Reen, R. K., Varadharaj, S., Khan, M., Childers, R. C., ... Gooch, K. J. (2015). Arterial levels of oxygen stimulate intimal hyperplasia in human saphenous veins via a ROS-dependent mechanism. PLoS One, 10(3), [e0120301]. https://doi.org/10.1371/journal.pone.0120301

Arterial levels of oxygen stimulate intimal hyperplasia in human saphenous veins via a ROS-dependent mechanism. / Joddar, Binata; Firstenberg, Michael S.; Reen, Rashmeet K.; Varadharaj, Saradhadevi; Khan, Mahmood; Childers, Rachel C.; Zweier, Jay L.; Gooch, Keith J.

In: PLoS One, Vol. 10, No. 3, e0120301, 23.03.2015.

Research output: Contribution to journalArticle

Joddar, B, Firstenberg, MS, Reen, RK, Varadharaj, S, Khan, M, Childers, RC, Zweier, JL & Gooch, KJ 2015, 'Arterial levels of oxygen stimulate intimal hyperplasia in human saphenous veins via a ROS-dependent mechanism', PLoS One, vol. 10, no. 3, e0120301. https://doi.org/10.1371/journal.pone.0120301
Joddar B, Firstenberg MS, Reen RK, Varadharaj S, Khan M, Childers RC et al. Arterial levels of oxygen stimulate intimal hyperplasia in human saphenous veins via a ROS-dependent mechanism. PLoS One. 2015 Mar 23;10(3). e0120301. https://doi.org/10.1371/journal.pone.0120301
Joddar, Binata ; Firstenberg, Michael S. ; Reen, Rashmeet K. ; Varadharaj, Saradhadevi ; Khan, Mahmood ; Childers, Rachel C. ; Zweier, Jay L. ; Gooch, Keith J. / Arterial levels of oxygen stimulate intimal hyperplasia in human saphenous veins via a ROS-dependent mechanism. In: PLoS One. 2015 ; Vol. 10, No. 3.
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