@inbook{8e140a8794074a299829a4475ead1b96,
title = "Array-based nuclear run-on analysis",
abstract = "There is extensive evidence that posttranscriptional mechanisms of gene regulation, such as mRNA turnover, critically affect the patterns of expressed mRNAs. Conventional microarray analysis measures steady-state messenger RNA (mRNA) levels, which represents the dynamic balance between new transcription and mRNA degradation. Accordingly, only de novo transcription can accurately reflect the temporal and spatial events of transcriptional regulation. In this chapter, we describe a recently reported method to study transcription systematically. It involves the genome-wide labeling of nascent transcripts using nonradioactive modified nucleotides, their isolation for amplification, and their hybridization and analysis using commercial microarrays.",
keywords = "Biotin, Microarray, Nascent RNA, Nuclear run-on, Posttranscriptional regulation",
author = "Jinshui Fan and Chen, {Yu Chi} and Tonya Watkins and Dang, {Chi V.} and Myriam Gorospe and Chris Cheadle",
year = "2012",
doi = "10.1007/978-1-61779-376-9_33",
language = "English (US)",
isbn = "9781617793752",
series = "Methods in Molecular Biology",
pages = "505--517",
editor = "Ales Vancura",
booktitle = "Transcriptional Regulation",
}