Are dopamine receptor and transporter changes in Rett syndrome reflected in Mecp2-deficient mice?

Dean Foster Wong, Mary E Blue, James R Brasic, Ayon Nandi, Heather Valentine, Kirstie H. Stansfield, Olivier Rousset, Genila M Bibat, Mary E. Yablonski, Michael V Johnston, Albert Gjedde, Sakkubai Naidu

Research output: Contribution to journalArticle

Abstract

We tested the claim that the dopaminergic dysfunction of Rett Syndrome (RTT) also occurs in Mecp2-deficient mice that serve as a model of the syndrome. We used positron emission tomography (PET) to image dopamine D2 receptors (D2R) and transporters (DAT) in women with RTT and in Mecp2-deficient mice, and D1R and D2R density was measured in postmortem human tissue by autoradiography. Results showed 1) significantly reduced D2R density in the striatum of women with RTT compared to control subjects. 2) PET imaging of mouse striatum similarly demonstrated significant reductions in D2R density of 7–10 week-old hemizygous (Mecp2-null) and heterozygous (HET) mice compared to wild type (WT) mice. With age, the density of D2R declined in WT mice but not HET mice. 3) In contrast, postmortem autoradiography revealed no group differences in the density of D1R and D2R in the caudate and putamen of RTT versus normal control subjects. 4) In humans and in the mouse model, PET revealed only marginal group differences in DAT. The results confirm that dopaminergic dysfunction in RTT is also present in Mecp2-deficient mice and that reductions in D2R more likely explain the impaired ambulation and progressive rigidity observed rather than alterations in DAT.

Original languageEnglish (US)
Pages (from-to)74-81
Number of pages8
JournalExperimental Neurology
Volume307
DOIs
StatePublished - Sep 1 2018

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Rett Syndrome
Dopamine Plasma Membrane Transport Proteins
Dopamine Receptors
Positron-Emission Tomography
Autoradiography
Dopamine D2 Receptors
Putamen
Walking

Keywords

  • Autoradiography
  • Binding potential
  • Gene mutation
  • Intellectual disability
  • Neurotransmitter
  • Nigrostriatal pathway
  • Partial volume correction
  • Positron emission tomography
  • Postmortem
  • Radioligand

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Are dopamine receptor and transporter changes in Rett syndrome reflected in Mecp2-deficient mice? / Wong, Dean Foster; Blue, Mary E; Brasic, James R; Nandi, Ayon; Valentine, Heather; Stansfield, Kirstie H.; Rousset, Olivier; Bibat, Genila M; Yablonski, Mary E.; Johnston, Michael V; Gjedde, Albert; Naidu, Sakkubai.

In: Experimental Neurology, Vol. 307, 01.09.2018, p. 74-81.

Research output: Contribution to journalArticle

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