Architecture of the Pontin/Reptin Complex, Essential in the Assembly of Several Macromolecular Complexes

Eva Torreira, Sudhakar Jha, José R. López-Blanco, Ernesto Arias-Palomo, Pablo Chacón, Cristina Cañas, Sylvia Ayora, Anindya Dutta, Oscar Llorca

Research output: Contribution to journalArticlepeer-review

Abstract

Pontin and reptin belong to the AAA+ family, and they are essential for the structural integrity and catalytic activity of several chromatin remodeling complexes. They are also indispensable for the assembly of several ribonucleoprotein complexes, including telomerase. Here, we propose a structural model of the yeast pontin/reptin complex based on a cryo-electron microscopy reconstruction at 13 Å. Pontin/reptin hetero-dodecamers were purified from in vivo assembled complexes forming a double ring. Two rings interact through flexible domains projecting from each hexamer, constituting an atypical asymmetric form of oligomerization. These flexible domains and the AAA+ cores reveal significant conformational changes when compared with the crystal structure of human pontin that generate enlarged channels. This structure of endogenously assembled pontin/reptin complexes is different than previously described structures, suggesting that pontin and reptin could acquire distinct structural states to regulate their broad functions as molecular motors and scaffolds for nucleic acids and proteins.

Original languageEnglish (US)
Pages (from-to)1511-1520
Number of pages10
JournalStructure
Volume16
Issue number10
DOIs
StatePublished - Oct 8 2008

Keywords

  • CELLBIO
  • PROTEINS

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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