Arc Oligomerization Is Regulated by CaMKII Phosphorylation of the GAG Domain: An Essential Mechanism for Plasticity and Memory Formation

Wenchi Zhang, Yang An Chuang, Youn Na, Zengyou Ye, Liuqing Yang, Raozhou Lin, Jiechao Zhou, Jing Wu, Jessica Qiu, Alena Savonenko, Daniel J. Leahy, Richard L Huganir, David J Linden, Paul F. Worley

Research output: Contribution to journalArticle

Abstract

Arc is a synaptic protein essential for memory consolidation. Recent studies indicate that Arc originates in evolution from a Ty3-Gypsy retrotransposon GAG domain. The N-lobe of Arc GAG domain acquired a hydrophobic binding pocket in higher vertebrates that is essential for Arc's canonical function to weaken excitatory synapses. Here, we report that Arc GAG also acquired phosphorylation sites that can acutely regulate its synaptic function. CaMKII phosphorylates the N-lobe of the Arc GAG domain and disrupts an interaction surface essential for high-order oligomerization. In Purkinje neurons, CaMKII phosphorylation acutely reverses Arc's synaptic action. Mutant Arc that cannot be phosphorylated by CaMKII enhances metabotropic receptor-dependent depression in the hippocampus but does not alter baseline synaptic transmission or long-term potentiation. Behavioral studies indicate that hippocampus- and amygdala-dependent learning requires Arc GAG domain phosphorylation. These studies provide an atomic model for dynamic and local control of Arc function underlying synaptic plasticity and memory. Normal learning and memory require dynamic control of synaptic connections. Arc plays a critical role in modifying synaptic strengths. Zhang et al. reveal that Arc's function is controlled by a novel regulatory mechanism that exploits its evolutionary origin as a retrotransposon GAG domain together with CaMKII that records activity history.

Original languageEnglish (US)
Pages (from-to)13-25.e5
JournalMolecular cell
Volume75
Issue number1
DOIs
StatePublished - Jul 11 2019

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 2
Phosphorylation
Retroelements
Hippocampus
Learning
Neuronal Plasticity
Long-Term Potentiation
Purkinje Cells
Amygdala
Synaptic Transmission
Synapses
Vertebrates
History
Depression
Proteins

Keywords

  • Arc
  • CAMKII
  • capsid
  • evolution
  • learning and memory
  • phosphorylation
  • polymerization
  • synapse plasticity

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Arc Oligomerization Is Regulated by CaMKII Phosphorylation of the GAG Domain : An Essential Mechanism for Plasticity and Memory Formation. / Zhang, Wenchi; Chuang, Yang An; Na, Youn; Ye, Zengyou; Yang, Liuqing; Lin, Raozhou; Zhou, Jiechao; Wu, Jing; Qiu, Jessica; Savonenko, Alena; Leahy, Daniel J.; Huganir, Richard L; Linden, David J; Worley, Paul F.

In: Molecular cell, Vol. 75, No. 1, 11.07.2019, p. 13-25.e5.

Research output: Contribution to journalArticle

Zhang, Wenchi ; Chuang, Yang An ; Na, Youn ; Ye, Zengyou ; Yang, Liuqing ; Lin, Raozhou ; Zhou, Jiechao ; Wu, Jing ; Qiu, Jessica ; Savonenko, Alena ; Leahy, Daniel J. ; Huganir, Richard L ; Linden, David J ; Worley, Paul F. / Arc Oligomerization Is Regulated by CaMKII Phosphorylation of the GAG Domain : An Essential Mechanism for Plasticity and Memory Formation. In: Molecular cell. 2019 ; Vol. 75, No. 1. pp. 13-25.e5.
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