Arachidonic acid-induced oxidative injury to cultured spinal cord neurons

Michal Toborek, Andrzej Malecki, Rosario Garrido, Mark P. Mattson, Bernhard Hennig, Byron Young

Research output: Contribution to journalArticle

Abstract

Spinal cord trauma can cause a marked release of free fatty acids, in particular, arachidonic acid (AA), from cell membranes. Free fatty acids, and AA by itself, may lead to secondary damage to spinal cord neurons. To study this hypothesis, cultured spinal cord neurons were exposed to increasing concentrations of AA (0.01-10 μM). AA-induced injury to spinal cord neurons was assessed by measurements of cellular oxidative stress, intracellular calcium levels, activation of nuclear factor-KB (NF-κB), and cell viability. AA treatment increased intracellular calcium concentrations and decreased cell viability. Oxidative stress increased significantly in neurons exposed to 1 and 10 μM AA. In addition, AA treatment activated NF-κB and decreased levels of the inhibitory subunit, IκB. It is interesting that manganese superoxide dismutase protein levels and levels of intracellular total glutathione increased in neurons exposed to this fatty acid for 24 h, consistent with a compensatory response to increased oxidative stress. These results strongly support the hypothesis that free fatty acids contribute to the tissue injury observed following spinal cord trauma.

Original languageEnglish (US)
Pages (from-to)684-692
Number of pages9
JournalJournal of Neurochemistry
Volume73
Issue number2
DOIs
StatePublished - 1999
Externally publishedYes

Keywords

  • Antioxidants
  • Free fatty acids
  • Oxidative stress
  • Spinal cord trauma

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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  • Cite this

    Toborek, M., Malecki, A., Garrido, R., Mattson, M. P., Hennig, B., & Young, B. (1999). Arachidonic acid-induced oxidative injury to cultured spinal cord neurons. Journal of Neurochemistry, 73(2), 684-692. https://doi.org/10.1046/j.1471-4159.1999.0730684.x