Arachidonic acid activates a proton current in the rat glutamate transporter EAAT4

Anastassios V. Tzingounis, Chien Liang Lin, Jeffrey D Rothstein, Michael P. Kavanaugh

Research output: Contribution to journalArticle

Abstract

The excitatory amino acid transporter EAAT4 is expressed predominantly in Purkinje neurons in the rat cerebellum (1-3), and it participates in postsynaptic re-uptake of glutamate released at the climbing fiber synapse (4). Transporter-mediated currents in Purkinje neurons are increased more than 3-fold by arachidonic acid, a second messenger that is liberated following depolarization-induced Ca2+ activation of phospholipase A2 (5). In this study we demonstrate that application of arachidonic acid to oocytes expressing rat EAAT4 increased glutamate-induced currents to a similar extent. However, arachidonic acid did not cause an increase in the rate of glutamate transport or in the chloride current associated with glutamate transport but rather activated a proton-selective conductance. These data reveal a novel action of arachidonate on a glutamate transporter and suggest a mechanism by which synaptic activity may decrease intracellular pH in neurons where this transporter is localized.

Original languageEnglish (US)
Pages (from-to)17315-17317
Number of pages3
JournalJournal of Biological Chemistry
Volume273
Issue number28
DOIs
StatePublished - Jul 10 1998

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Amino Acid Transport System X-AG
Arachidonic Acid
Protons
Rats
Glutamic Acid
Neurons
Purkinje Cells
Amino Acid Transport Systems
Excitatory Amino Acids
Phospholipases A2
Induced currents
Depolarization
Second Messenger Systems
Synapses
Cerebellum
Oocytes
Chlorides
Chemical activation
Fibers

ASJC Scopus subject areas

  • Biochemistry

Cite this

Arachidonic acid activates a proton current in the rat glutamate transporter EAAT4. / Tzingounis, Anastassios V.; Lin, Chien Liang; Rothstein, Jeffrey D; Kavanaugh, Michael P.

In: Journal of Biological Chemistry, Vol. 273, No. 28, 10.07.1998, p. 17315-17317.

Research output: Contribution to journalArticle

Tzingounis, Anastassios V. ; Lin, Chien Liang ; Rothstein, Jeffrey D ; Kavanaugh, Michael P. / Arachidonic acid activates a proton current in the rat glutamate transporter EAAT4. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 28. pp. 17315-17317.
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