Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut

Audrey E. Koid, Martin D. Chapman, Robert G. Hamilton, Ronald Van Ree, Serge A. Versteeg, Stephen C. Dreskin, Stef J. Koppelman, Sabina Wünschmann

Research output: Contribution to journalArticlepeer-review

Abstract

The similarities of two major peanut allergens, Ara h 2 and Ara h 6, in molecular size, amino acid sequence, and structure have made it difficult to obtain natural Ara h 6 free of Ara h 2. The objectives of this study were to purify natural Ara h 6 that is essentially free of Ara h 2 and to compare its IgE reactivity and potency in histamine release assays to Ara h 2. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the highly purified allergen (<0.01% Ara h 2) revealed a single 14.5 kD band, and the identity of Ara h 6 was confirmed by liquid chromatography-tandem mass spectrometry. Ara h 6 showed a higher seroprevalence in chimeric IgE enzyme-linked immunosorbent assay (n = 54) but a weaker biological activity in basophil histamine release assays than Ara h 2. Purified Ara h 6 will be useful for diagnostic IgE antibody assays as well as molecular and cellular studies to investigate the immunological mechanisms of peanut allergy.

Original languageEnglish (US)
Pages (from-to)206-213
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number1
DOIs
StatePublished - Jan 8 2014

Keywords

  • IgE reactivity
  • allergen
  • histamine release
  • peanut

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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