Aqueous to cornea fluorescein distribution ratio in normal and swollen cornea

John S. Minkowski, Mitsuru Sawa, Stephen P. Bartels, Kenneth R. Kenyon, Arthur H. Neufeld

Research output: Contribution to journalArticlepeer-review

Abstract

Intravitreal sodium fluorescein was used to simulate equilibrium fluorescein kinetics, thereby allowing simple measurement of the aqueous to cornea fluorescein distribution ratio. Two groups of rabbit corneas were studied: normal corneas and corneas wounded by freezing. The aqueous to cornea fluorescein distribution ratio was approximately 0.4, was not significantly different in groups of normal or wounded eyes and little variability was noted. In addition, a comparison of in vivo and in vitro measurements of corneal fluorescein concentration in wounded eyes suggests that in vivo protein-bound fluorescein in the cornea fluoresces less efficiently than free fluorescein.

Original languageEnglish (US)
Pages (from-to)156-159
Number of pages4
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume224
Issue number2
DOIs
StatePublished - Mar 1 1986

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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