Aqueous to cornea fluorescein distribution ratio in normal and swollen cornea

John S. Minkowski; Mitsuru Sawa; Stephen P. Bartels; Kenneth R. Kenyon; Arthur H. Neufeld

doi:10.1007/BF02141490

Aqueous to cornea fluorescein distribution ratio in normal and swollen cornea

John S. Minkowski, Mitsuru Sawa, Stephen P. Bartels, Kenneth R. Kenyon, Arthur H. Neufeld

School of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Intravitreal sodium fluorescein was used to simulate equilibrium fluorescein kinetics, thereby allowing simple measurement of the aqueous to cornea fluorescein distribution ratio. Two groups of rabbit corneas were studied: normal corneas and corneas wounded by freezing. The aqueous to cornea fluorescein distribution ratio was approximately 0.4, was not significantly different in groups of normal or wounded eyes and little variability was noted. In addition, a comparison of in vivo and in vitro measurements of corneal fluorescein concentration in wounded eyes suggests that in vivo protein-bound fluorescein in the cornea fluoresces less efficiently than free fluorescein.

Original language	English (US)
Pages (from-to)	156-159
Number of pages	4
Journal	Graefe's Archive for Clinical and Experimental Ophthalmology
Volume	224
Issue number	2
DOIs	https://doi.org/10.1007/BF02141490
State	Published - Mar 1 1986

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "Aqueous to cornea fluorescein distribution ratio in normal and swollen cornea",

abstract = "Intravitreal sodium fluorescein was used to simulate equilibrium fluorescein kinetics, thereby allowing simple measurement of the aqueous to cornea fluorescein distribution ratio. Two groups of rabbit corneas were studied: normal corneas and corneas wounded by freezing. The aqueous to cornea fluorescein distribution ratio was approximately 0.4, was not significantly different in groups of normal or wounded eyes and little variability was noted. In addition, a comparison of in vivo and in vitro measurements of corneal fluorescein concentration in wounded eyes suggests that in vivo protein-bound fluorescein in the cornea fluoresces less efficiently than free fluorescein.",

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AU - Minkowski, John S.

AU - Sawa, Mitsuru

AU - Bartels, Stephen P.

AU - Kenyon, Kenneth R.

AU - Neufeld, Arthur H.

PY - 1986/3/1

Y1 - 1986/3/1

N2 - Intravitreal sodium fluorescein was used to simulate equilibrium fluorescein kinetics, thereby allowing simple measurement of the aqueous to cornea fluorescein distribution ratio. Two groups of rabbit corneas were studied: normal corneas and corneas wounded by freezing. The aqueous to cornea fluorescein distribution ratio was approximately 0.4, was not significantly different in groups of normal or wounded eyes and little variability was noted. In addition, a comparison of in vivo and in vitro measurements of corneal fluorescein concentration in wounded eyes suggests that in vivo protein-bound fluorescein in the cornea fluoresces less efficiently than free fluorescein.

AB - Intravitreal sodium fluorescein was used to simulate equilibrium fluorescein kinetics, thereby allowing simple measurement of the aqueous to cornea fluorescein distribution ratio. Two groups of rabbit corneas were studied: normal corneas and corneas wounded by freezing. The aqueous to cornea fluorescein distribution ratio was approximately 0.4, was not significantly different in groups of normal or wounded eyes and little variability was noted. In addition, a comparison of in vivo and in vitro measurements of corneal fluorescein concentration in wounded eyes suggests that in vivo protein-bound fluorescein in the cornea fluoresces less efficiently than free fluorescein.

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Aqueous to cornea fluorescein distribution ratio in normal and swollen cornea

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