Aquaporins in Saccharomyces: Genetic and functional distinctions between laboratory and wild-type strains

Mélanie Bonhivers, Jennifer M. Carbrey, Stephen J. Gould, Peter Agre

Research output: Contribution to journalArticlepeer-review

Abstract

Aquaporin water channel proteins mediate the transport of water across cell membranes in numerous species. The Saccharomyces genome data base contains an open reading frame (here designated AQY1) that encodes a protein with strong homology to aquaporins. AQY1 from laboratory and wild-type strains of Saccharomyces were expressed in Xenopus oocytes to determine the coefficients of osmotic water permeability (P(f)). Oocytes injected with wild-type AQY1 cRNAs exhibit high P(f) values, whereas oocytes injected with AQY1 cRNAs from laboratory strains exhibit low P(f) values and have reduced levels of Aqy1p due to two amino acid substitutions. When the AQY1 gene was deleted from a wild-type yeast and cells were cultured in vitro with cycled hypo-osmolar or hyper-osmolar stresses, the AQY1 null yeast showed significantly improved viability when compared with the parental wild-type strain. We conclude that Saccharomyces cerevisiae contains at least one aquaporin gene, but it is not functional in laboratory strains due to apparent negative selection pressures resulting from in vitro methods.

Original languageEnglish (US)
Pages (from-to)27565-27572
Number of pages8
JournalJournal of Biological Chemistry
Volume273
Issue number42
DOIs
StatePublished - Oct 16 1998

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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