TY - JOUR
T1 - Aquaporins in Saccharomyces
T2 - Genetic and functional distinctions between laboratory and wild-type strains
AU - Bonhivers, Mélanie
AU - Carbrey, Jennifer M.
AU - Gould, Stephen J.
AU - Agre, Peter
PY - 1998/10/16
Y1 - 1998/10/16
N2 - Aquaporin water channel proteins mediate the transport of water across cell membranes in numerous species. The Saccharomyces genome data base contains an open reading frame (here designated AQY1) that encodes a protein with strong homology to aquaporins. AQY1 from laboratory and wild-type strains of Saccharomyces were expressed in Xenopus oocytes to determine the coefficients of osmotic water permeability (P(f)). Oocytes injected with wild-type AQY1 cRNAs exhibit high P(f) values, whereas oocytes injected with AQY1 cRNAs from laboratory strains exhibit low P(f) values and have reduced levels of Aqy1p due to two amino acid substitutions. When the AQY1 gene was deleted from a wild-type yeast and cells were cultured in vitro with cycled hypo-osmolar or hyper-osmolar stresses, the AQY1 null yeast showed significantly improved viability when compared with the parental wild-type strain. We conclude that Saccharomyces cerevisiae contains at least one aquaporin gene, but it is not functional in laboratory strains due to apparent negative selection pressures resulting from in vitro methods.
AB - Aquaporin water channel proteins mediate the transport of water across cell membranes in numerous species. The Saccharomyces genome data base contains an open reading frame (here designated AQY1) that encodes a protein with strong homology to aquaporins. AQY1 from laboratory and wild-type strains of Saccharomyces were expressed in Xenopus oocytes to determine the coefficients of osmotic water permeability (P(f)). Oocytes injected with wild-type AQY1 cRNAs exhibit high P(f) values, whereas oocytes injected with AQY1 cRNAs from laboratory strains exhibit low P(f) values and have reduced levels of Aqy1p due to two amino acid substitutions. When the AQY1 gene was deleted from a wild-type yeast and cells were cultured in vitro with cycled hypo-osmolar or hyper-osmolar stresses, the AQY1 null yeast showed significantly improved viability when compared with the parental wild-type strain. We conclude that Saccharomyces cerevisiae contains at least one aquaporin gene, but it is not functional in laboratory strains due to apparent negative selection pressures resulting from in vitro methods.
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U2 - 10.1074/jbc.273.42.27565
DO - 10.1074/jbc.273.42.27565
M3 - Article
C2 - 9765289
AN - SCOPUS:0032538552
SN - 0021-9258
VL - 273
SP - 27565
EP - 27572
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 42
ER -