Aquaporin in Candida: Characterization of a functional water channel protein

Jennifer M. Carbrey, Brendan P. Cormack, Peter Agre

Research output: Contribution to journalArticle

Abstract

The Candida albicans genome database contains one ORF with homology to aquaporins, AQY1. Xenopus oocytes injected with cRNA encoding C. albicans Aqy1p displayed a coefficient of water permeability (Pf) that was equivalent to the Pf for oocytes injected with the cRNA of S. cerevisiae Aqy1p. In addition, as seen in Saccharomyces for Aqy1p and Aqy2p, deletion of AQY1 in C. albicans resulted in cells that were less sensitive than wild-type to osmotic shock. In Saccharomyces, aquaporin null cells also have a cell surface that is more hydrophobic. However, unlike Saccharomyces, there was no effect on the cell surface hydrophobicity, flocculation or cell aggregation in aqy1 null C. albicans cells. Perhaps as a result, there was no difference between the virulence of C. albicans wild-type and aqy1 null strains in a murine model for systemic candidiasis.

Original languageEnglish (US)
Pages (from-to)1391-1396
Number of pages6
JournalYeast
Volume18
Issue number15
DOIs
StatePublished - Dec 5 2001

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Keywords

  • Aquaporin
  • Candida albicans
  • Water channel
  • Yeast

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

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