Aqp1 expression in erythroleukemia cells: Genetic regulation of glucocorticoid and chemical induction

Research output: Contribution to journalArticlepeer-review

Abstract

The aquaporin-1 (AQP1) water channel protein is expressed in multiple mammalian tissues by several different developmental programs; however, the genetic regulation is undefined. The proximal promoter of mouse Aqp1 contains multiple putative cis-acting regulatory elements, and mouse erythroleukemia (MEL) cells are a well-characterized model for erythroid differentiation. Corticosteroid or dimethyl sulfoxide (DMSO) exposure induces AQP1 protein expression in MEL cells, and transcriptional regulation was investigated by transient transfections with Aqp1 promoter-reporter constructs. Dexamethasone induction is abrogated by deletion of two glucocorticoid response elements - 0.5 kilobases (kb) from the transcription initiation site. Mutation of the GATA element at -0.62 kb has no effect, whereas mutation of the CACCC site at -37 bp significantly reduces DMSO-induced promoter activity. Hydroxyurea induces expression of AQP1 protein without acting through the proximal promoter. The MEL cell line is a reproducible erythroid model system for studying transcriptional regulation of the Aqp1 gene while determining the consequences on AQP1 protein biosynthesis.

Original languageEnglish (US)
Pages (from-to)C1562-C1570
JournalAmerican Journal of Physiology - Cell Physiology
Volume273
Issue number5 42-5
DOIs
StatePublished - 1997

Keywords

  • Aquaporin-1
  • Erythroid tissue
  • Promoter
  • Protein biosynthesis
  • Transcriptional regulation
  • Water channels

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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