Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts

Richard B. Sheridan, P. C. Huang

Research output: Contribution to journalArticlepeer-review

Abstract

The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 ± 12 units vs. 214 ± 28 units) towards apurinic and/or apyrimidinic sites as determined in a "nicking" assay.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume52
Issue number1
DOIs
StatePublished - Oct 1978

Keywords

  • BSA
  • EDTA
  • HEPES
  • N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid
  • SDS
  • bovine serum albumin
  • ethylenediamine tetraacetic acid
  • sodium dodecylsulfate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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