Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts

Richard B. Sheridan; P. C. Huang

doi:10.1016/0027-5107(78)90101-X

Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts

Richard B. Sheridan, P. C. Huang

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 ± 12 units vs. 214 ± 28 units) towards apurinic and/or apyrimidinic sites as determined in a "nicking" assay.

Original language	English (US)
Pages (from-to)	129-136
Number of pages	8
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	52
Issue number	1
DOIs	https://doi.org/10.1016/0027-5107(78)90101-X
State	Published - Oct 1978

Keywords

BSA
EDTA
HEPES
N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid
SDS
bovine serum albumin
ethylenediamine tetraacetic acid
sodium dodecylsulfate

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

Access to Document

10.1016/0027-5107(78)90101-X

Fingerprint Dive into the research topics of 'Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts'. Together they form a unique fingerprint.

Cite this

@article{d26dceb264164ad18a4b4efc499e187e,

title = "Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts",

abstract = "The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 ± 12 units vs. 214 ± 28 units) towards apurinic and/or apyrimidinic sites as determined in a {"}nicking{"} assay.",

keywords = "BSA, EDTA, HEPES, N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid, SDS, bovine serum albumin, ethylenediamine tetraacetic acid, sodium dodecylsulfate",

author = "Sheridan, {Richard B.} and Huang, {P. C.}",

year = "1978",

month = oct,

doi = "10.1016/0027-5107(78)90101-X",

language = "English (US)",

volume = "52",

pages = "129--136",

journal = "Mutation Research",

issn = "0027-5107",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts

AU - Sheridan, Richard B.

AU - Huang, P. C.

PY - 1978/10

Y1 - 1978/10

N2 - The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 ± 12 units vs. 214 ± 28 units) towards apurinic and/or apyrimidinic sites as determined in a "nicking" assay.

AB - The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 ± 12 units vs. 214 ± 28 units) towards apurinic and/or apyrimidinic sites as determined in a "nicking" assay.

KW - BSA

KW - EDTA

KW - HEPES

KW - N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid

KW - SDS

KW - bovine serum albumin

KW - ethylenediamine tetraacetic acid

KW - sodium dodecylsulfate

UR - http://www.scopus.com/inward/record.url?scp=0017846647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017846647&partnerID=8YFLogxK

U2 - 10.1016/0027-5107(78)90101-X

DO - 10.1016/0027-5107(78)90101-X

M3 - Article

C2 - 215905

AN - SCOPUS:0017846647

VL - 52

SP - 129

EP - 136

JO - Mutation Research

JF - Mutation Research

SN - 0027-5107

IS - 1

ER -