Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts

Richard B. Sheridan; P. C. Huang

doi:10.1016/0027-5107(78)90101-X

Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts

Richard B. Sheridan, P. C. Huang

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 ± 12 units vs. 214 ± 28 units) towards apurinic and/or apyrimidinic sites as determined in a "nicking" assay.

Original language	English (US)
Pages (from-to)	129-136
Number of pages	8
Journal	Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume	52
Issue number	1
DOIs	https://doi.org/10.1016/0027-5107(78)90101-X
State	Published - Oct 1978
Externally published	Yes

Keywords

BSA
EDTA
HEPES
N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid
SDS
bovine serum albumin
ethylenediamine tetraacetic acid
sodium dodecylsulfate

ASJC Scopus subject areas

Molecular Biology
Genetics
Health, Toxicology and Mutagenesis

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10.1016/0027-5107(78)90101-X

Cite this

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title = "Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts",

abstract = "The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 ± 12 units vs. 214 ± 28 units) towards apurinic and/or apyrimidinic sites as determined in a {"}nicking{"} assay.",

keywords = "BSA, EDTA, HEPES, N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid, SDS, bovine serum albumin, ethylenediamine tetraacetic acid, sodium dodecylsulfate",

author = "Sheridan, {Richard B.} and Huang, {P. C.}",

note = "Funding Information: This study was supported by grants from the National Foundation (March of Dimes) CRBS-300 and NIH ROLES01596, ES2PO100454. A preliminary report was presented before the 5th International Congress on Birth Defects, Montreal (Canada) on August 26, 1977. We thank Drs. Roger McMacken and Nancy Shaper for valuable comments.",

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doi = "10.1016/0027-5107(78)90101-X",

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pages = "129--136",

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T1 - Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts

AU - Sheridan, Richard B.

AU - Huang, P. C.

N1 - Funding Information: This study was supported by grants from the National Foundation (March of Dimes) CRBS-300 and NIH ROLES01596, ES2PO100454. A preliminary report was presented before the 5th International Congress on Birth Defects, Montreal (Canada) on August 26, 1977. We thank Drs. Roger McMacken and Nancy Shaper for valuable comments.

PY - 1978/10

Y1 - 1978/10

N2 - The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 ± 12 units vs. 214 ± 28 units) towards apurinic and/or apyrimidinic sites as determined in a "nicking" assay.

AB - The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 ± 12 units vs. 214 ± 28 units) towards apurinic and/or apyrimidinic sites as determined in a "nicking" assay.

KW - BSA

KW - EDTA

KW - HEPES

KW - N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid

KW - SDS

KW - bovine serum albumin

KW - ethylenediamine tetraacetic acid

KW - sodium dodecylsulfate

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AN - SCOPUS:0017846647

SN - 0027-5107

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JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

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IS - 1

ER -

Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts

Abstract

Keywords

ASJC Scopus subject areas

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