Approaches to bacterial RNA isolation and purification for microarray analysis of Escherichia coli K1 interaction with human brain microvascular endothelial cells

Francescopaolo Di Cello, Yi Xie, Maneesh Paul-Satyaseela, Kwang Sik Kim

Research output: Contribution to journalArticlepeer-review

Abstract

We established a protocol for isolation of microarray-grade bacterial RNA from Escherichia coli K1 interacting with human brain microvascular endothelial cells. The extracted RNA was free of human RNA contamination. More importantly, microarray analysis demonstrated that no bias was introduced in the gene expression pattern during the RNA isolation procedure.

Original languageEnglish (US)
Pages (from-to)4197-4199
Number of pages3
JournalJournal of clinical microbiology
Volume43
Issue number8
DOIs
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Microbiology (medical)

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