Abstract
Inflammatory bowel diseases (IBD) are chronic, heterogeneous, and multifactorial intestinal inflammatory disorders. Major challenges in IBD research include identification of major pathogenic alterations of genes/proteins as well as effective biomarkers for early diagnosis, prognosis, and prediction of therapeutic response. Since proteins govern cellular structure and biological function, a wide selection of proteomic approaches enables effective characterization of IBD pathogenesis by investigating the dynamic nature of protein expression, cellular and subcellular distribution, posttranslational modifications, and interactions at both the cellular and subcellular levels. The aims of this review are to 1) highlight the current status of proteomic studies of IBD, and 2) introduce the available and emerging proteomic technologies that have potential applications in the study of IBD. These technologies include various mass spectrometry technologies, quantitative proteomics (2D-PAGE, ICAT, SILAC, iTRAQ), protein/antibody arrays, and multiepitope-ligand cartography. This review also presents information and methodologies, from sample selection and enrichment to protein identification, that are not only essential but also particularly relevant to IBD research. The potential future application of these technologies is expected to have a significant impact on the discovery of novel biomarkers and key pathogenic factors for IBD.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 616-629 |
| Number of pages | 14 |
| Journal | Inflammatory Bowel Diseases |
| Volume | 15 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2009 |
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Keywords
- Biomarkers
- Crohn's disease
- Inflammatory bowel disease (IBD)
- Proteomics
- Ulcerative colitis
ASJC Scopus subject areas
- Gastroenterology
- Immunology and Allergy
Cite this
Applications of proteomics in the study of inflammatory bowel diseases : Current status and future directions with available technologies. / Alex, Philip; Gucek, Marjan; Li, Xuhang.
In: Inflammatory Bowel Diseases, Vol. 15, No. 4, 2009, p. 616-629.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Applications of proteomics in the study of inflammatory bowel diseases
T2 - Current status and future directions with available technologies
AU - Alex, Philip
AU - Gucek, Marjan
AU - Li, Xuhang
PY - 2009
Y1 - 2009
N2 - Inflammatory bowel diseases (IBD) are chronic, heterogeneous, and multifactorial intestinal inflammatory disorders. Major challenges in IBD research include identification of major pathogenic alterations of genes/proteins as well as effective biomarkers for early diagnosis, prognosis, and prediction of therapeutic response. Since proteins govern cellular structure and biological function, a wide selection of proteomic approaches enables effective characterization of IBD pathogenesis by investigating the dynamic nature of protein expression, cellular and subcellular distribution, posttranslational modifications, and interactions at both the cellular and subcellular levels. The aims of this review are to 1) highlight the current status of proteomic studies of IBD, and 2) introduce the available and emerging proteomic technologies that have potential applications in the study of IBD. These technologies include various mass spectrometry technologies, quantitative proteomics (2D-PAGE, ICAT, SILAC, iTRAQ), protein/antibody arrays, and multiepitope-ligand cartography. This review also presents information and methodologies, from sample selection and enrichment to protein identification, that are not only essential but also particularly relevant to IBD research. The potential future application of these technologies is expected to have a significant impact on the discovery of novel biomarkers and key pathogenic factors for IBD.
AB - Inflammatory bowel diseases (IBD) are chronic, heterogeneous, and multifactorial intestinal inflammatory disorders. Major challenges in IBD research include identification of major pathogenic alterations of genes/proteins as well as effective biomarkers for early diagnosis, prognosis, and prediction of therapeutic response. Since proteins govern cellular structure and biological function, a wide selection of proteomic approaches enables effective characterization of IBD pathogenesis by investigating the dynamic nature of protein expression, cellular and subcellular distribution, posttranslational modifications, and interactions at both the cellular and subcellular levels. The aims of this review are to 1) highlight the current status of proteomic studies of IBD, and 2) introduce the available and emerging proteomic technologies that have potential applications in the study of IBD. These technologies include various mass spectrometry technologies, quantitative proteomics (2D-PAGE, ICAT, SILAC, iTRAQ), protein/antibody arrays, and multiepitope-ligand cartography. This review also presents information and methodologies, from sample selection and enrichment to protein identification, that are not only essential but also particularly relevant to IBD research. The potential future application of these technologies is expected to have a significant impact on the discovery of novel biomarkers and key pathogenic factors for IBD.
KW - Biomarkers
KW - Crohn's disease
KW - Inflammatory bowel disease (IBD)
KW - Proteomics
KW - Ulcerative colitis
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UR - http://www.scopus.com/inward/citedby.url?scp=67650189497&partnerID=8YFLogxK
U2 - 10.1002/ibd.20652
DO - 10.1002/ibd.20652
M3 - Article
C2 - 18844215
AN - SCOPUS:67650189497
VL - 15
SP - 616
EP - 629
JO - Inflammatory Bowel Diseases
JF - Inflammatory Bowel Diseases
SN - 1078-0998
IS - 4
ER -