Application of statistical experimental designs for the optimization of medium constituents for the production of citric acid from pineapple waste

Sarat Babu Imandi, Veera Venkata Ratnam Bandaru, Subba Rao Somalanka, Sita Ramalakshmi Bandaru, Hanumantha Rao Garapati

Research output: Contribution to journalArticle

Abstract

Statistical experimental designs were applied for the optimization of medium constituents for citric acid production by Yarrowia lipolytica NCIM 3589 in solid state fermentation (SSF) using pineapple waste as the sole substrate. Using Plackett-Burman design, yeast extract, moisture content of the substrate, KH2PO4 and Na2HPO4 were identified as significant variables which highly influenced citric acid production and these variables were subsequently optimized using a central composite design (CCD). The optimum conditions were found to be yeast extract 0.34 (%w/w), moisture content of the substrate 70.71 (%), KH2PO4 0.64 (%w/w) and Na2HPO4 0.69 (%w/w). Citric acid production at these optimum conditions was 202.35 g/kg ds (g citric acid produced/kg of dried pineapple waste as substrate).

Original languageEnglish (US)
Pages (from-to)4445-4450
Number of pages6
JournalBioresource Technology
Volume99
Issue number10
DOIs
StatePublished - Jul 1 2008

Keywords

  • Central composite design
  • Citric acid
  • Pineapple waste
  • Plackett-Burman design
  • Yarrowia lipolytica

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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