Application of natural partial digests to pulsed-field gel analysis of the amplified MDR locus

Eckart Meese, Paul Meltzer, Jeffrey Trent

Research output: Contribution to journalArticle

Abstract

The analysis by pulsed-field gel electrophoresis of partial digestion products visualized by probes for the human multidrug resistance (MDR) locus has been used to further establish the restriction map of this region. Results place the MDR1 and MDR2 genes on a single SfiI fragment, with partial digestion products establishing the distance between these genes to be 230-250 kb. The feasibility and potential advantages of using "natural" partials to generate detailed restriction maps within an amplified DNA domain are discussed.

Original languageEnglish (US)
Pages (from-to)371-374
Number of pages4
JournalGenomics
Volume5
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Multiple Drug Resistance
Digestion
Gels
Pulsed Field Gel Electrophoresis
Genes
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Application of natural partial digests to pulsed-field gel analysis of the amplified MDR locus. / Meese, Eckart; Meltzer, Paul; Trent, Jeffrey.

In: Genomics, Vol. 5, No. 2, 1989, p. 371-374.

Research output: Contribution to journalArticle

Meese, Eckart ; Meltzer, Paul ; Trent, Jeffrey. / Application of natural partial digests to pulsed-field gel analysis of the amplified MDR locus. In: Genomics. 1989 ; Vol. 5, No. 2. pp. 371-374.
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