Application of meta-analysis methods for identifying proteomic expression level differences

Bob Amess, Wolfgang Kluge, Emanuel Schwarz, Frieder Haenisch, Murtada Alsaif, Robert H Yolken, F. Markus Leweke, Paul C. Guest, Sabine Bahn

Research output: Contribution to journalArticle

Abstract

We present new statistical approaches for identification of proteins with expression levels that are significantly changed when applying meta-analysis to two or more independent experiments. We showed that the Euclidean distance measure has reduced risk of false positives compared to the rank product method. Our Ψ-ranking method has advantages over the traditional fold-change approach by incorporating both the fold-change direction as well as the p-value. In addition, the second novel method, Π-ranking, considers the ratio of the fold-change and thus integrates all three parameters. We further improved the latter by introducing our third technique, Σ-ranking, which combines all three parameters in a balanced nonparametric approach.

Original languageEnglish (US)
Pages (from-to)2072-2076
Number of pages5
JournalProteomics
Volume13
Issue number14
DOIs
StatePublished - Jul 2013

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Proteomics
Meta-Analysis
Proteins
Experiments
Direction compound

Keywords

  • Bipolar disorder
  • Fold-change
  • Meta analysis
  • Statistics
  • Technology

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Amess, B., Kluge, W., Schwarz, E., Haenisch, F., Alsaif, M., Yolken, R. H., ... Bahn, S. (2013). Application of meta-analysis methods for identifying proteomic expression level differences. Proteomics, 13(14), 2072-2076. https://doi.org/10.1002/pmic.201300034

Application of meta-analysis methods for identifying proteomic expression level differences. / Amess, Bob; Kluge, Wolfgang; Schwarz, Emanuel; Haenisch, Frieder; Alsaif, Murtada; Yolken, Robert H; Leweke, F. Markus; Guest, Paul C.; Bahn, Sabine.

In: Proteomics, Vol. 13, No. 14, 07.2013, p. 2072-2076.

Research output: Contribution to journalArticle

Amess, B, Kluge, W, Schwarz, E, Haenisch, F, Alsaif, M, Yolken, RH, Leweke, FM, Guest, PC & Bahn, S 2013, 'Application of meta-analysis methods for identifying proteomic expression level differences', Proteomics, vol. 13, no. 14, pp. 2072-2076. https://doi.org/10.1002/pmic.201300034
Amess, Bob ; Kluge, Wolfgang ; Schwarz, Emanuel ; Haenisch, Frieder ; Alsaif, Murtada ; Yolken, Robert H ; Leweke, F. Markus ; Guest, Paul C. ; Bahn, Sabine. / Application of meta-analysis methods for identifying proteomic expression level differences. In: Proteomics. 2013 ; Vol. 13, No. 14. pp. 2072-2076.
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