Application of HR-MAS NMR spectroscopy for studying chemotype variations of Withania somnifera (L.) Dunal

S. K. Bharti, Anil Bhatia, S. K. Tewari, O. P. Sidhu, Raja Roy

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Withania somnifera (L.) Dunal (Solanaceae), commonly known as Ashwagandha, is one of the most valued Indian medicinal plants with a number of pharmaceutical and nutraceutical applications. Metabolic profiling has been performed by HR-MAS NMR spectroscopy on fresh leaf and root tissue specimens from four chemotypes of W. somnifera. The HR-MAS NMR spectroscopy of lyophilized defatted leaf tissue specimens clearly distinguishes resonances of medicinally important secondary metabolites (withaferin A and withanone) and its distinctive quantitative variability among the chemotypes. A total of 41 metabolites were identified from both the leaf and root tissues of the chemotypes. The presence of methanol in leaf and root tissues of W. somnifera was detected by HR-MAS NMR spectroscopy. Multivariate principal component analysis (PCA) on HR-MAS 1H NMR spectra of leaves revealed clear variations in primary metabolites among the chemotypes. The results of the present study demonstrated an efficient method, which can be utilized for metabolite profiling of primary and secondary metabolites in medicinally important plants.

Original languageEnglish (US)
Pages (from-to)659-667
Number of pages9
JournalMagnetic Resonance in Chemistry
Issue number10
StatePublished - Oct 1 2011
Externally publishedYes


  • Withania somnifera
  • metabolic profile
  • principal component analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)


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