Application of flow cytometry for the assessment of preservation and recovery efficiency of bioaerosol samplers spiked with Pantoea agglomerans

Ana M Rule, Jana Kesavan, Kellogg Schwab, Timothy J. Buckley

Research output: Contribution to journalArticle

Abstract

Exposure assessment of biological aerosols requires trade-offs between efficient sampling of airborne microorganisms as either particles or viable units. The main objective of this work was to characterize aspects of bioaerosol measurement efficiency. A known concentration of the vegetative bacteria Pantoea agglomerans was spiked onto different samplers (AGI-30, BioSampler, and membrane filters) and then run for increasing time periods using HEPA filtered air. Measurement efficiency was evaluated based on total, viable, and culturable counts. Total and viable counts were determined by flow-cytometry (FCM); culturable counts were evaluated by standard plating. FCM as a method for assaying viability showed excellent agreement with known proportions of live/dead organisms (slope = 0.82, R2 = 0.99). P. agglomerans recoveries (total, viable, and culturable) in order of best sampler performance included the BioSampler (75%, 52%, and 50%), filtration (50%, 13%, and 2%), and the AGI-30 (

Original languageEnglish (US)
Pages (from-to)2467-2472
Number of pages6
JournalEnvironmental Science and Technology
Volume41
Issue number7
DOIs
StatePublished - Apr 1 2007

Fingerprint

efficiency measurement
Flow cytometry
flow cytometry
sampler
Recovery
Aerosols
Plating
Microorganisms
Bacteria
viability
microorganism
aerosol
Sampling
membrane
filter
Membranes
bacterium
sampling
air
Air

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Application of flow cytometry for the assessment of preservation and recovery efficiency of bioaerosol samplers spiked with Pantoea agglomerans. / Rule, Ana M; Kesavan, Jana; Schwab, Kellogg; Buckley, Timothy J.

In: Environmental Science and Technology, Vol. 41, No. 7, 01.04.2007, p. 2467-2472.

Research output: Contribution to journalArticle

@article{750dbd9880af4783bae9ab2c8339710f,
title = "Application of flow cytometry for the assessment of preservation and recovery efficiency of bioaerosol samplers spiked with Pantoea agglomerans",
abstract = "Exposure assessment of biological aerosols requires trade-offs between efficient sampling of airborne microorganisms as either particles or viable units. The main objective of this work was to characterize aspects of bioaerosol measurement efficiency. A known concentration of the vegetative bacteria Pantoea agglomerans was spiked onto different samplers (AGI-30, BioSampler, and membrane filters) and then run for increasing time periods using HEPA filtered air. Measurement efficiency was evaluated based on total, viable, and culturable counts. Total and viable counts were determined by flow-cytometry (FCM); culturable counts were evaluated by standard plating. FCM as a method for assaying viability showed excellent agreement with known proportions of live/dead organisms (slope = 0.82, R2 = 0.99). P. agglomerans recoveries (total, viable, and culturable) in order of best sampler performance included the BioSampler (75{\%}, 52{\%}, and 50{\%}), filtration (50{\%}, 13{\%}, and 2{\%}), and the AGI-30 (",
author = "Rule, {Ana M} and Jana Kesavan and Kellogg Schwab and Buckley, {Timothy J.}",
year = "2007",
month = "4",
day = "1",
doi = "10.1021/es062394l",
language = "English (US)",
volume = "41",
pages = "2467--2472",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - Application of flow cytometry for the assessment of preservation and recovery efficiency of bioaerosol samplers spiked with Pantoea agglomerans

AU - Rule, Ana M

AU - Kesavan, Jana

AU - Schwab, Kellogg

AU - Buckley, Timothy J.

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Exposure assessment of biological aerosols requires trade-offs between efficient sampling of airborne microorganisms as either particles or viable units. The main objective of this work was to characterize aspects of bioaerosol measurement efficiency. A known concentration of the vegetative bacteria Pantoea agglomerans was spiked onto different samplers (AGI-30, BioSampler, and membrane filters) and then run for increasing time periods using HEPA filtered air. Measurement efficiency was evaluated based on total, viable, and culturable counts. Total and viable counts were determined by flow-cytometry (FCM); culturable counts were evaluated by standard plating. FCM as a method for assaying viability showed excellent agreement with known proportions of live/dead organisms (slope = 0.82, R2 = 0.99). P. agglomerans recoveries (total, viable, and culturable) in order of best sampler performance included the BioSampler (75%, 52%, and 50%), filtration (50%, 13%, and 2%), and the AGI-30 (

AB - Exposure assessment of biological aerosols requires trade-offs between efficient sampling of airborne microorganisms as either particles or viable units. The main objective of this work was to characterize aspects of bioaerosol measurement efficiency. A known concentration of the vegetative bacteria Pantoea agglomerans was spiked onto different samplers (AGI-30, BioSampler, and membrane filters) and then run for increasing time periods using HEPA filtered air. Measurement efficiency was evaluated based on total, viable, and culturable counts. Total and viable counts were determined by flow-cytometry (FCM); culturable counts were evaluated by standard plating. FCM as a method for assaying viability showed excellent agreement with known proportions of live/dead organisms (slope = 0.82, R2 = 0.99). P. agglomerans recoveries (total, viable, and culturable) in order of best sampler performance included the BioSampler (75%, 52%, and 50%), filtration (50%, 13%, and 2%), and the AGI-30 (

UR - http://www.scopus.com/inward/record.url?scp=34247144673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247144673&partnerID=8YFLogxK

U2 - 10.1021/es062394l

DO - 10.1021/es062394l

M3 - Article

C2 - 17438801

AN - SCOPUS:34247144673

VL - 41

SP - 2467

EP - 2472

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 7

ER -