Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis

David Calligaris, Diana Caragacianu, Xiaohui Liu, Isaiah Norton, Christopher J. Thompson, Andrea L. Richardson, Mehra Golshan, Michael L. Easterling, Sandro Santagata, Deborah A. Dillon, Ferenc A. Jolesz, Nathalie Y.R. Agar

Research output: Contribution to journalArticlepeer-review

Abstract

Distinguishing tumor from normal glandular breast tissue is an important step in breast-conserving surgery. Because this distinction can be challenging in the operative setting, up to 40% of patients require an additional operation when traditional approaches are used. Here,we present a proof-of-concept study to determine the feasibility of using desorption electrospray ionization mass spectrometry imaging (DESI-MSI) for identifying and differentiating tumor from normal breast tissue.We show that tumor margins can be identified using the spatial distributions and varying intensities of different lipids. Several fatty acids, including oleic acid, were more abundant in the cancerous tissue than in normal tissues. The cancer margins delineated by the molecular images from DESI-MSI were consistent with thosemargins obtained fromhistological staining. Our findings prove the feasibility of classifying cancerous and normal breast tissues using ambient ionization MSI. The results suggest that an MS-based method could be developed for the rapid intraoperative detection of residual cancer tissue during breast-conserving surgery.

Original languageEnglish (US)
Pages (from-to)15184-15189
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number42
DOIs
StatePublished - Oct 21 2014
Externally publishedYes

Keywords

  • FT-ICR MS
  • Intrasurgical diagnosis
  • Metabolites
  • Molecular pathology

ASJC Scopus subject areas

  • General

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