Application of Coherent Anti-stokes Raman Scattering (CARS) to imaging mammalian cells: A means for gaining molecular selectivity in multiphoton imaging

Gary Holtom, Brian Thrall, Thomas Weber, Leyun Zhu, Derek Hopkins, Christopher Parkinson, Steven Colson, John Price, Beek Yoke Chin, Augustine Choi, Terence Risby

Research output: Contribution to journalArticle

Abstract

Coherent anti-Stokes Raman scattering (CARS) was employed to provide molecular selectivity in the imaging of mammalian cells. The method involved multiphoton fluorescence achieved by using ultrafast laser pulses. The addition of a second laser beam increased the detected Raman signal by many orders of magnitude besides removing fluorescence artifacts. An optical parametric amplifier and confocal microscopy was also utilized for the imaging process.

Original languageEnglish (US)
Pages (from-to)319-328
Number of pages10
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4262
DOIs
StatePublished - Jan 1 2001

Keywords

  • CARS
  • Confocal microscopy
  • Multiphoton fluorescence
  • Raman imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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