Application of Chromatography Combined With Mass Spectrometry in Therapeutic Drug Monitoring

Madhuri Manohar; Mark A Marzinke

doi:10.1016/B978-0-12-802025-8.00003-9

Application of Chromatography Combined With Mass Spectrometry in Therapeutic Drug Monitoring

Madhuri Manohar, Mark A Marzinke

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Liquid chromatography-mass spectrometry (LC-MS) is a technology of emerging importance in the field of therapeutic drug monitoring (TDM) because development of commercial immunoassays for new TDM applications is sparse at best. LC-MS approaches have shown tremendous potential in the field of TDM. Assays are highly sensitive and selective, and can overcome challenges associated with conventional immunoassays. However, there are limitations and caveats to be considered when implementing this technology into the clinical laboratory environment. A review of methodological approaches, applications, advantages, and limitations will be discussed in this chapter.

Original language	English (US)
Title of host publication	Clinical Challenges in Therapeutic Drug Monitoring: Special Populations, Physiological Conditions, and Pharmacogenomics
Publisher	Elsevier Inc.
Pages	45-70
Number of pages	26
ISBN (Electronic)	9780128020524
ISBN (Print)	9780128020258
DOIs	https://doi.org/10.1016/B978-0-12-802025-8.00003-9
State	Published - May 17 2016

Keywords

Immunoassays
Ion suppression
Liquid chromatography
Mass spectrometry
Positive bias

ASJC Scopus subject areas

General Medicine

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10.1016/B978-0-12-802025-8.00003-9

Cite this

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abstract = "Liquid chromatography-mass spectrometry (LC-MS) is a technology of emerging importance in the field of therapeutic drug monitoring (TDM) because development of commercial immunoassays for new TDM applications is sparse at best. LC-MS approaches have shown tremendous potential in the field of TDM. Assays are highly sensitive and selective, and can overcome challenges associated with conventional immunoassays. However, there are limitations and caveats to be considered when implementing this technology into the clinical laboratory environment. A review of methodological approaches, applications, advantages, and limitations will be discussed in this chapter.",

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N2 - Liquid chromatography-mass spectrometry (LC-MS) is a technology of emerging importance in the field of therapeutic drug monitoring (TDM) because development of commercial immunoassays for new TDM applications is sparse at best. LC-MS approaches have shown tremendous potential in the field of TDM. Assays are highly sensitive and selective, and can overcome challenges associated with conventional immunoassays. However, there are limitations and caveats to be considered when implementing this technology into the clinical laboratory environment. A review of methodological approaches, applications, advantages, and limitations will be discussed in this chapter.

AB - Liquid chromatography-mass spectrometry (LC-MS) is a technology of emerging importance in the field of therapeutic drug monitoring (TDM) because development of commercial immunoassays for new TDM applications is sparse at best. LC-MS approaches have shown tremendous potential in the field of TDM. Assays are highly sensitive and selective, and can overcome challenges associated with conventional immunoassays. However, there are limitations and caveats to be considered when implementing this technology into the clinical laboratory environment. A review of methodological approaches, applications, advantages, and limitations will be discussed in this chapter.

KW - Immunoassays

KW - Ion suppression

KW - Liquid chromatography

KW - Mass spectrometry

KW - Positive bias

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M3 - Chapter

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BT - Clinical Challenges in Therapeutic Drug Monitoring: Special Populations, Physiological Conditions, and Pharmacogenomics

PB - Elsevier Inc.

ER -

Application of Chromatography Combined With Mass Spectrometry in Therapeutic Drug Monitoring

Abstract

Keywords

ASJC Scopus subject areas

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