Application of Chromatography Combined With Mass Spectrometry in Therapeutic Drug Monitoring

Madhuri Manohar, Mark A Marzinke

Research output: Chapter in Book/Report/Conference proceedingChapter


Liquid chromatography-mass spectrometry (LC-MS) is a technology of emerging importance in the field of therapeutic drug monitoring (TDM) because development of commercial immunoassays for new TDM applications is sparse at best. LC-MS approaches have shown tremendous potential in the field of TDM. Assays are highly sensitive and selective, and can overcome challenges associated with conventional immunoassays. However, there are limitations and caveats to be considered when implementing this technology into the clinical laboratory environment. A review of methodological approaches, applications, advantages, and limitations will be discussed in this chapter.

Original languageEnglish (US)
Title of host publicationClinical Challenges in Therapeutic Drug Monitoring: Special Populations, Physiological Conditions, and Pharmacogenomics
PublisherElsevier Inc.
Number of pages26
ISBN (Electronic)9780128020524
ISBN (Print)9780128020258
StatePublished - May 17 2016


  • Immunoassays
  • Ion suppression
  • Liquid chromatography
  • Mass spectrometry
  • Positive bias

ASJC Scopus subject areas

  • Medicine(all)


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