Application of carbon monoxide diffusing capacity in the mouse lung

Jon Fallica, Sandhya Das, Maureen Horton, Wayne A Mitzner

Research output: Contribution to journalArticle

Abstract

In the past decade the mouse has become the primary animal model of a variety of lung diseases. To assess various mechanisms underlying such pathologies, it is essential to make functional measurements that can reflect the developing pathology. In this regard, the diffusing capacity for carbon monoxide is a variable that directly reflects structural changes in the lung. Although measurement of single-breath diffusing capacity of the lung for carbon monoxide (DLCO) has also been previously reported in mice by a number of investigators, a number of technical issues have precluded routine and widespread use of this metric in mouse models. In the present report, we describe a means to quickly and simply measure a dimensionless variable closely related to the DLCO in mice, termed a diffusion factor for carbon monoxide (DFCO). The DFCO procedure involves a 9-s lung inflation with tracer gases in an anesthetized mouse, followed by a 1-min gas analysis time. We have tested the approach with two common models of lung pathology, elastase-induced emphysema and bleomycin-induced fibrosis. Results show a significant 15% reduction in DFCO in emphysema, and a 41% reduction in the fibrosis model. Repeat measurements within a mouse were found to be highly reproducible. This pulmonary function test can thus be used to detect structural changes with these pathological models. The method can also be used to measure changes in pulmonary blood volume, since the uptake of CO is highly dependent on this variable in addition to the gas exchange surface area.

Original languageEnglish (US)
Pages (from-to)1455-1459
Number of pages5
JournalJournal of Applied Physiology
Volume110
Issue number5
DOIs
StatePublished - May 2011

Fingerprint

Carbon Monoxide
Lung
Gases
Emphysema
Pathology
Fibrosis
Lung Volume Measurements
Pancreatic Elastase
Respiratory Function Tests
Bleomycin
Economic Inflation
Blood Volume
Lung Diseases
Animal Models
Research Personnel

Keywords

  • Diffusion
  • Gas exchange
  • Structure-function

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Application of carbon monoxide diffusing capacity in the mouse lung. / Fallica, Jon; Das, Sandhya; Horton, Maureen; Mitzner, Wayne A.

In: Journal of Applied Physiology, Vol. 110, No. 5, 05.2011, p. 1455-1459.

Research output: Contribution to journalArticle

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