Application of a collagen-based membrane and chondroitin sulfate-based hydrogel adhesive for the potential repair of severe ocular surface injuries

Jemin J. Chae, Daniel G. Mulreany, Qiongyu Guo, Qiaozhi Lu, Joseph S. Choi, Iossif Strehin, Freddy A. Espinoza, Oliver Schein, Morgana M. Trexler, Kraig S. Bower, Jennifer H. Elisseeff

Research output: Contribution to journalArticlepeer-review

Abstract

This study was performed to evaluate the potential of a chondroitin sulfate-polyethylene glycol (CS-PEG) adhesive and collagen-based membrane (collagen vitrigel, CV) combination as a method to treat penetrating ocular injuries on the battlefield and to improve this method with two technologies: an antibiotic releasing CS-PEG adhesive and a corneal shaped CV. Burst testing using porcine cadaveric eyes, high-performance liquid chromatography, the Kirby-Bauer bacterial inhibition test, and CV implantations on the live and cadaveric rabbit eyes were performed. The ocular burst test showed CS-PEG adhesive could successfully repair 5-mm to 6-mm length wounds in the corneal and corneoscleral regions but would require CS-PEG + CV to treat larger wounds similar to those seen on the battlefield. In addition, high performance liquid chromatography and the Kirby-Bauer bacterial inhibition test presented evidence suggesting the vancomycin incorporated CS-PEG could inhibit Staphylococcus infection for 9 days. Furthermore, the curved CV showed an advantage by matching the corneal contour without any wrinkle formation. Although this pilot study showed a limited range of possible applications, we demonstrated that the combination of CS-PEG adhesive + CV is a promising method and the 2 technologies improve their applicability to the special demands of the battlefield.

Original languageEnglish (US)
Pages (from-to)686-694
Number of pages9
JournalMilitary medicine
Volume179
Issue number6
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

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