Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella bacteremia

Kevin Jeng; Samuel Yang; Helen Won; Charlotte A. Gaydos; Yu Hsiang Hsieh; Alex Kecojevic; Karen C. Carroll; Justin Hardick; Richard E. Rothmana

doi:10.1128/JCM.05121-11

Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella bacteremia

Kevin Jeng, Samuel Yang, Helen Won, Charlotte A. Gaydos, Yu Hsiang Hsieh, Alex Kecojevic, Karen C. Carroll, Justin Hardick, Richard E. Rothmana

School of Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.

Original language	English (US)
Pages (from-to)	1122-1124
Number of pages	3
Journal	Journal of clinical microbiology
Volume	50
Issue number	3
DOIs	https://doi.org/10.1128/JCM.05121-11
State	Published - Mar 2012

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.05121-11

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title = "Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella bacteremia",

abstract = "Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.",

author = "Kevin Jeng and Samuel Yang and Helen Won and Gaydos, {Charlotte A.} and Hsieh, {Yu Hsiang} and Alex Kecojevic and Carroll, {Karen C.} and Justin Hardick and Rothmana, {Richard E.}",

year = "2012",

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AU - Jeng, Kevin

AU - Yang, Samuel

AU - Won, Helen

AU - Gaydos, Charlotte A.

AU - Hsieh, Yu Hsiang

AU - Kecojevic, Alex

AU - Carroll, Karen C.

AU - Hardick, Justin

AU - Rothmana, Richard E.

PY - 2012/3

Y1 - 2012/3

N2 - Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.

AB - Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.

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Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella bacteremia

Abstract

ASJC Scopus subject areas

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