Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein

Gregory M. Preston, Tiziana Piazza Carroll, William B Guggino, Peter C Agre

Research output: Contribution to journalArticle

Abstract

Water rapidly crosses the plasma membrane of red blood cells (RBCs) and renal tubules through specialized channels. Although selective for water, the molecular structure of these channels is unknown. The CHIP28 protein is an abundant integral membrane protein in mammalian RBCs and renal proximal tubules and belongs to a family of membrane proteins with unknown functions. Oocytes from Xenopus laevis microinjected with in vitro-transcribed CHIP28 RNA exhibited increased osmotic water permeability; this was reversibly inhibited by mercuric chloride, a known inhibitor of water channels. Therefore it is likely that CHIP28 is a functional unit of membrane water channels.

Original languageEnglish (US)
Pages (from-to)385-387
Number of pages3
JournalScience
Volume256
Issue number5055
StatePublished - Apr 17 1992

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Aquaporin 1
Aquaporins
Xenopus
Oocytes
Water
Membrane Proteins
Erythrocytes
Mercuric Chloride
Proximal Kidney Tubule
Xenopus laevis
Molecular Structure
Ion Channels
Permeability
Cell Membrane
RNA
Kidney

ASJC Scopus subject areas

  • General

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Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. / Preston, Gregory M.; Carroll, Tiziana Piazza; Guggino, William B; Agre, Peter C.

In: Science, Vol. 256, No. 5055, 17.04.1992, p. 385-387.

Research output: Contribution to journalArticle

Preston, Gregory M. ; Carroll, Tiziana Piazza ; Guggino, William B ; Agre, Peter C. / Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. In: Science. 1992 ; Vol. 256, No. 5055. pp. 385-387.
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