TY - JOUR
T1 - Apoptotic Photoreceptor Cell Death After Traumatic Retinal Detachment in Humans
AU - Chang, Cheng Jong
AU - Lai, Wico W.
AU - Edward, Deepak P.
AU - Tso, Mark O.M.
N1 - Funding Information:
ThisstudywassupportedinpartbyNationalEye Instituteresearch 06761,AmeliorationofReti¬ nalPhotic Injury, andgrantsEY01903, Pathology of Retinal Dys¬ function;coregrantEY01792fromtheNationalEyeIn¬ stitute, Bethesda, Md; giftsfrom the Lions ofIllinois Foundation, Hillside; andan unrestricted research grant fromResearchtoPreventBlindnessIne,NewYork,NY. DrChangissupportedinpartbytheTri-serviceGeneral HospitalandNationalDefenseMedicalCenter, Taipei,Tai¬ wan, Republic of China. Dr Tso is a Research to Prevent BlindnessSeniorScientificInvestigatorandisalsoonleave as Professor ofOphthalmology, Chinese University ofHong Kong.sored MrtheLaiAmericanis a medicalHeartstudentAssociationresearchand fellowwill be spon¬start¬
PY - 1995/7
Y1 - 1995/7
N2 - Objective: To determine the mechanism of photoreceptor cell death after traumatic retinal detachment in humans. Design: Clinical records from 1975 to 1993 of 75 patients, whose eyes were enucleated after traumatic retinal detachment, were reviewed for age, sex, previous ocular or systemic medical history, interval from initial trauma to enucleation, visual acuity, and types of trauma. The patients were divided into five groups of 15 cases each, based on the interval from initial trauma to enucleation. The retinal tissue was examined for two markers of apoptosis: (1) nicked nuclear DNA in situ by the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) technique and (2) apoptotic bodies by light and electron microscopy. Results: Of the 75 cases of ruptured globe and traumatic retinal detachment that were evaluated, 19 eyes (25.3%) showed TUNEL-positive labeling of photoreceptor cells. Nicked nuclear DNA was detected in photoreceptor cells of detached retinas as early as 8 hours after trauma. The detached retinas in seven of 15 eyes enucleated within 2 days after ocular trauma showed TUNEL-positive photoreceptor nuclei. The number of cases showing TUNEL-positive photoreceptor nuclei decreased as the interval between initial trauma and enucleation increased. The TUNEL-positive photoreceptor cells could still be seen in the detached retinas of two eyes enucleated 22 days after trauma. Light microscopy disclosed condensation and fragmentation of photoreceptor nuclei in the detached retinas. Electron microscopy showed structures resembling apoptotic bodies phagocytosed by neighboring cells in the TUNEL-positive retinas. Conclusions: Apoptosis is an important mechanism of photoreceptor cell degeneration in the early stage after traumatic retinal detachment in humans.
AB - Objective: To determine the mechanism of photoreceptor cell death after traumatic retinal detachment in humans. Design: Clinical records from 1975 to 1993 of 75 patients, whose eyes were enucleated after traumatic retinal detachment, were reviewed for age, sex, previous ocular or systemic medical history, interval from initial trauma to enucleation, visual acuity, and types of trauma. The patients were divided into five groups of 15 cases each, based on the interval from initial trauma to enucleation. The retinal tissue was examined for two markers of apoptosis: (1) nicked nuclear DNA in situ by the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) technique and (2) apoptotic bodies by light and electron microscopy. Results: Of the 75 cases of ruptured globe and traumatic retinal detachment that were evaluated, 19 eyes (25.3%) showed TUNEL-positive labeling of photoreceptor cells. Nicked nuclear DNA was detected in photoreceptor cells of detached retinas as early as 8 hours after trauma. The detached retinas in seven of 15 eyes enucleated within 2 days after ocular trauma showed TUNEL-positive photoreceptor nuclei. The number of cases showing TUNEL-positive photoreceptor nuclei decreased as the interval between initial trauma and enucleation increased. The TUNEL-positive photoreceptor cells could still be seen in the detached retinas of two eyes enucleated 22 days after trauma. Light microscopy disclosed condensation and fragmentation of photoreceptor nuclei in the detached retinas. Electron microscopy showed structures resembling apoptotic bodies phagocytosed by neighboring cells in the TUNEL-positive retinas. Conclusions: Apoptosis is an important mechanism of photoreceptor cell degeneration in the early stage after traumatic retinal detachment in humans.
UR - http://www.scopus.com/inward/record.url?scp=0028791777&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028791777&partnerID=8YFLogxK
U2 - 10.1001/archopht.1995.01100070054025
DO - 10.1001/archopht.1995.01100070054025
M3 - Article
C2 - 7605279
AN - SCOPUS:0028791777
SN - 0003-9950
VL - 113
SP - 880
EP - 886
JO - Archives of ophthalmology
JF - Archives of ophthalmology
IS - 7
ER -