Apoptosis parallels lymphopoiesis in bone marrow transplantation and HIV disease

A. D. Donnenberg, J. B. Margolick, L. A. Beltz, V. S. Donnenberg, C. R. Rinaldo

Research output: Contribution to journalArticlepeer-review

Abstract

Apoptosis has been implicated in a variety of physiological processes ranging from tissue modeling to deletion of autoreactive T lymphocytes during thymic development. The recent finding that a large proportion of peripheral T cells from HIV-infected subjets apoptose in culture raises an important issue: does this represent a pathologic mechanism by which the virus disrupts the immune system, or a normal physiologic response to virus-mediated T-cell loss? To study the potential relationship between apoptosis and lymphopoiesis, we compared apoptosis rates in unstimulated lymphocyte cultures from healthy subjets, HIV+ gay men, and bone marrow transplant (BMT) recipients undergoing immune reconstitution. BMT recipients were chosen because they undergo massive regeneration of lymphocytes following marrow ablation and graft infusion. The data obtained in BMT recipients suggests that elevated apoptosis accompanies, and is the consequence of, elevated lymphopoiesis. We also found a strong inverse relationship between in vitro T-cell apoptosis rates and peripheral T-cell counts. These results provide a new interpretation for elevated apoptosis observed in HIV-infected individuals - that it reflects increased T-cell turnover consequent to virus-mediated destruction of CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)11-21
Number of pages11
JournalResearch in Immunology
Volume146
Issue number1
DOIs
StatePublished - Jan 1995

Keywords

  • AIDS
  • Apoptosis
  • Bone marrow
  • Flow Cytometry
  • HIV
  • Lymphopoiesis
  • Tolerance
  • Transplantation

ASJC Scopus subject areas

  • Immunology

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