Apoptosis leads to photoreceptor degeneration in inherited retinal dystrophy of RCS rats

M. O.M. Tso, C. Zhang, A. S. Abler, C. J. Chang, F. Wong, G. Q. Chang, T. T. Lam

Research output: Contribution to journalArticle

Abstract

Purpose. To determine the pathogenetic mechanism of photoreceptor cell degeneration in the inherited retinal dystrophy in Royal College of Surgeons (RCS) rats. Methods. The dystrophic retinas of the pink-eyed RCS (RCS-rdy-p) rats were examined for DNA fragmentation by agarose gel electrophoresis of retinal DNA and by TdT-mediated biotin-dUDP nick-end labeling (TUNEL) in paraffin sections. Rats ranging in age from 3 to 60 days were examined. Results. Agarose gel electrophoresis of retinal DNA isolated from animals 25, 30, 35, and 40 days old showed a ladder pattern of degradation with bands corresponding to multiples of 180 to 200 base pair subunits. TUNEL study showed increasing labeling of photoreceptor cells with progression of the retinal dystrophy of the RCS rats. Conclusions. Apoptosis is the dominant mechanism of photoreceptor degeneration in the RCS rat, which has a genetic defect in the phagocytic activity of retinal pigment epithelium. The onset of the degeneration appeared to vary between rod cells in the different regions of the eye.

Original languageEnglish (US)
Pages (from-to)2693-2699
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume35
Issue number6
StatePublished - Jan 1 1994

Keywords

  • RCS rats
  • apoptosis
  • photo-receptor cells
  • programmed cell death
  • retinal dystrophy

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Tso, M. O. M., Zhang, C., Abler, A. S., Chang, C. J., Wong, F., Chang, G. Q., & Lam, T. T. (1994). Apoptosis leads to photoreceptor degeneration in inherited retinal dystrophy of RCS rats. Investigative Ophthalmology and Visual Science, 35(6), 2693-2699.