Apoptosis in the Neuronal Lineage of the Mouse Olfactory Epithelium: Regulationin Vivoandin Vitro

J. David Holcomb, Jeffrey Mumm, Anne L. Calof

Research output: Contribution to journalArticle

Abstract

The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical removal of one olfactory bulb, the synaptic target of olfactory receptor neurons (ORNs) of the OE); and in primary cell cultures derived from embryonic mouse OE.In vivo,cells at all stages in the neuronal lineage-proliferating neuronal precursors, immature ORNs, and mature ORNs-displayed signs of apoptotic cell death; nonneuronal cells did not. Bulbectomy dramatically increased the number of apoptotic cells in the OE on the bulbectomized side. Shortly following bulbectomy, increased cell death involved neuronal cells of all stages. Later, cell death remained persistently elevated, but this was due to increased apoptosis by mature ORNs alone.In vitro,apoptotic death of both ORNs and their precursors could be inhibited by agents that prevent apoptosis in other cells: aurintricarboxylic acid (ATA), a membrane-permeant analog of cyclic AMP (CPT-cAMP), and certain members of the neurotrophin family of growth factors (brain-derived neurotrophic factor, neurotrophin 3, and neurotrophin 5), although no neurotrophin was as effective at promoting survival as ATA or CPT-cAMP. Consistent with observed effects of neurotrophins, immunohistochemistry localized the neurotrophin receptors trkB and trkC to fractions of ORNs scattered throughout neonatal OE. These results suggest that apoptosis may regulate neuronal number in the OE at multiple stages in the neuronal lineage and that multiple factors-potentially including certain neurotrophins-may be involved in this process.

Original languageEnglish (US)
Pages (from-to)307-323
Number of pages17
JournalDevelopmental Biology
Volume172
Issue number1
DOIs
StatePublished - Nov 1995
Externally publishedYes

Fingerprint

Olfactory Mucosa
Olfactory Receptor Neurons
Apoptosis
Nerve Growth Factors
Cell Death
Aurintricarboxylic Acid
Neurotrophin 3
Nerve Growth Factor Receptors
Primary Cell Culture
Olfactory Bulb
Brain-Derived Neurotrophic Factor
Cyclic AMP
Regeneration
Intercellular Signaling Peptides and Proteins
Cell Count
Immunohistochemistry
Parturition
Neurons
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Apoptosis in the Neuronal Lineage of the Mouse Olfactory Epithelium : Regulationin Vivoandin Vitro. / Holcomb, J. David; Mumm, Jeffrey; Calof, Anne L.

In: Developmental Biology, Vol. 172, No. 1, 11.1995, p. 307-323.

Research output: Contribution to journalArticle

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