Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes

Lalita Gupta, Ju Young Noh, Yong Hun Jo, Seung Han Oh, Sanjeev Kumar, Mi Young Noh, Yong Seok Lee, Sung-Jae Cha, Sook Jae Seo, Iksoo Kim, Yeon Soo Han, Carolina Barillas-Mury

Research output: Contribution to journalArticle

Abstract

Background: Apolipophorin-III (ApoLp-III) is known to play an important role in lipid transport and innate immunity in lepidopteran insects. However, there is no evidence of involvement of ApoLp-IIIs in the immune responses of dipteran insects such as Drosophila and mosquitoes. Methodology/Principal Findings: We report the molecular and functional characterization of An. gambiae apolipophorin-III (AgApoLp-III). Mosquito ApoLp-IIIs have diverged extensively from those of lepidopteran insects; however, the predicted tertiary structure of AgApoLp-III is similar to that of Manduca sexta (tobacco hornworm). We found that AgApoLp-III mRNA expression is strongly induced in the midgut of An. gambiae (G3 strain) mosquitoes in response to Plasmodium berghei infection. Furthermore, immunofluorescence stainings revealed that high levels of AgApoLp-III protein accumulate in the cytoplasm of Plasmodium-invaded cells and AgApoLp-III silencing increases the intensity of P. berghei infection by five fold. Conclusion: There are broad differences in the midgut epithelial responses to Plasmodium invasion between An. gambiae strains. In the G3 strain of An. gambiae AgApoLp-III participates in midgut epithelial defense responses that limit Plasmodium infection.

Original languageEnglish (US)
Article numbere15410
JournalPLoS One
Volume5
Issue number11
DOIs
StatePublished - 2010

Fingerprint

apolipophorin
Anopheles gambiae
antimalarials
Culicidae
Plasmodium
midgut
Malaria
Plasmodium berghei
Manduca
Insects
Manduca sexta
insects
Tobacco
Lepidoptera
infection
apolipophorin III
Innate Immunity
fluorescent antibody technique
Drosophila
Fluorescent Antibody Technique

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Gupta, L., Noh, J. Y., Jo, Y. H., Oh, S. H., Kumar, S., Noh, M. Y., ... Barillas-Mury, C. (2010). Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes. PLoS One, 5(11), [e15410]. https://doi.org/10.1371/journal.pone.0015410

Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes. / Gupta, Lalita; Noh, Ju Young; Jo, Yong Hun; Oh, Seung Han; Kumar, Sanjeev; Noh, Mi Young; Lee, Yong Seok; Cha, Sung-Jae; Seo, Sook Jae; Kim, Iksoo; Han, Yeon Soo; Barillas-Mury, Carolina.

In: PLoS One, Vol. 5, No. 11, e15410, 2010.

Research output: Contribution to journalArticle

Gupta, L, Noh, JY, Jo, YH, Oh, SH, Kumar, S, Noh, MY, Lee, YS, Cha, S-J, Seo, SJ, Kim, I, Han, YS & Barillas-Mury, C 2010, 'Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes', PLoS One, vol. 5, no. 11, e15410. https://doi.org/10.1371/journal.pone.0015410
Gupta, Lalita ; Noh, Ju Young ; Jo, Yong Hun ; Oh, Seung Han ; Kumar, Sanjeev ; Noh, Mi Young ; Lee, Yong Seok ; Cha, Sung-Jae ; Seo, Sook Jae ; Kim, Iksoo ; Han, Yeon Soo ; Barillas-Mury, Carolina. / Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes. In: PLoS One. 2010 ; Vol. 5, No. 11.
@article{34aa9364e71541528646d205a094f90d,
title = "Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes",
abstract = "Background: Apolipophorin-III (ApoLp-III) is known to play an important role in lipid transport and innate immunity in lepidopteran insects. However, there is no evidence of involvement of ApoLp-IIIs in the immune responses of dipteran insects such as Drosophila and mosquitoes. Methodology/Principal Findings: We report the molecular and functional characterization of An. gambiae apolipophorin-III (AgApoLp-III). Mosquito ApoLp-IIIs have diverged extensively from those of lepidopteran insects; however, the predicted tertiary structure of AgApoLp-III is similar to that of Manduca sexta (tobacco hornworm). We found that AgApoLp-III mRNA expression is strongly induced in the midgut of An. gambiae (G3 strain) mosquitoes in response to Plasmodium berghei infection. Furthermore, immunofluorescence stainings revealed that high levels of AgApoLp-III protein accumulate in the cytoplasm of Plasmodium-invaded cells and AgApoLp-III silencing increases the intensity of P. berghei infection by five fold. Conclusion: There are broad differences in the midgut epithelial responses to Plasmodium invasion between An. gambiae strains. In the G3 strain of An. gambiae AgApoLp-III participates in midgut epithelial defense responses that limit Plasmodium infection.",
author = "Lalita Gupta and Noh, {Ju Young} and Jo, {Yong Hun} and Oh, {Seung Han} and Sanjeev Kumar and Noh, {Mi Young} and Lee, {Yong Seok} and Sung-Jae Cha and Seo, {Sook Jae} and Iksoo Kim and Han, {Yeon Soo} and Carolina Barillas-Mury",
year = "2010",
doi = "10.1371/journal.pone.0015410",
language = "English (US)",
volume = "5",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes

AU - Gupta, Lalita

AU - Noh, Ju Young

AU - Jo, Yong Hun

AU - Oh, Seung Han

AU - Kumar, Sanjeev

AU - Noh, Mi Young

AU - Lee, Yong Seok

AU - Cha, Sung-Jae

AU - Seo, Sook Jae

AU - Kim, Iksoo

AU - Han, Yeon Soo

AU - Barillas-Mury, Carolina

PY - 2010

Y1 - 2010

N2 - Background: Apolipophorin-III (ApoLp-III) is known to play an important role in lipid transport and innate immunity in lepidopteran insects. However, there is no evidence of involvement of ApoLp-IIIs in the immune responses of dipteran insects such as Drosophila and mosquitoes. Methodology/Principal Findings: We report the molecular and functional characterization of An. gambiae apolipophorin-III (AgApoLp-III). Mosquito ApoLp-IIIs have diverged extensively from those of lepidopteran insects; however, the predicted tertiary structure of AgApoLp-III is similar to that of Manduca sexta (tobacco hornworm). We found that AgApoLp-III mRNA expression is strongly induced in the midgut of An. gambiae (G3 strain) mosquitoes in response to Plasmodium berghei infection. Furthermore, immunofluorescence stainings revealed that high levels of AgApoLp-III protein accumulate in the cytoplasm of Plasmodium-invaded cells and AgApoLp-III silencing increases the intensity of P. berghei infection by five fold. Conclusion: There are broad differences in the midgut epithelial responses to Plasmodium invasion between An. gambiae strains. In the G3 strain of An. gambiae AgApoLp-III participates in midgut epithelial defense responses that limit Plasmodium infection.

AB - Background: Apolipophorin-III (ApoLp-III) is known to play an important role in lipid transport and innate immunity in lepidopteran insects. However, there is no evidence of involvement of ApoLp-IIIs in the immune responses of dipteran insects such as Drosophila and mosquitoes. Methodology/Principal Findings: We report the molecular and functional characterization of An. gambiae apolipophorin-III (AgApoLp-III). Mosquito ApoLp-IIIs have diverged extensively from those of lepidopteran insects; however, the predicted tertiary structure of AgApoLp-III is similar to that of Manduca sexta (tobacco hornworm). We found that AgApoLp-III mRNA expression is strongly induced in the midgut of An. gambiae (G3 strain) mosquitoes in response to Plasmodium berghei infection. Furthermore, immunofluorescence stainings revealed that high levels of AgApoLp-III protein accumulate in the cytoplasm of Plasmodium-invaded cells and AgApoLp-III silencing increases the intensity of P. berghei infection by five fold. Conclusion: There are broad differences in the midgut epithelial responses to Plasmodium invasion between An. gambiae strains. In the G3 strain of An. gambiae AgApoLp-III participates in midgut epithelial defense responses that limit Plasmodium infection.

UR - http://www.scopus.com/inward/record.url?scp=78149477892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78149477892&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0015410

DO - 10.1371/journal.pone.0015410

M3 - Article

C2 - 21072214

AN - SCOPUS:78149477892

VL - 5

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e15410

ER -