Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes

Lalita Gupta, Ju Young Noh, Yong Hun Jo, Seung Han Oh, Sanjeev Kumar, Mi Young Noh, Yong Seok Lee, Sung Jae Cha, Sook Jae Seo, Iksoo Kim, Yeon Soo Han, Carolina Barillas-Mury

Research output: Contribution to journalArticle

Abstract

Background: Apolipophorin-III (ApoLp-III) is known to play an important role in lipid transport and innate immunity in lepidopteran insects. However, there is no evidence of involvement of ApoLp-IIIs in the immune responses of dipteran insects such as Drosophila and mosquitoes. Methodology/Principal Findings: We report the molecular and functional characterization of An. gambiae apolipophorin-III (AgApoLp-III). Mosquito ApoLp-IIIs have diverged extensively from those of lepidopteran insects; however, the predicted tertiary structure of AgApoLp-III is similar to that of Manduca sexta (tobacco hornworm). We found that AgApoLp-III mRNA expression is strongly induced in the midgut of An. gambiae (G3 strain) mosquitoes in response to Plasmodium berghei infection. Furthermore, immunofluorescence stainings revealed that high levels of AgApoLp-III protein accumulate in the cytoplasm of Plasmodium-invaded cells and AgApoLp-III silencing increases the intensity of P. berghei infection by five fold. Conclusion: There are broad differences in the midgut epithelial responses to Plasmodium invasion between An. gambiae strains. In the G3 strain of An. gambiae AgApoLp-III participates in midgut epithelial defense responses that limit Plasmodium infection.

Original languageEnglish (US)
Article numbere15410
JournalPloS one
Volume5
Issue number11
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Gupta, L., Noh, J. Y., Jo, Y. H., Oh, S. H., Kumar, S., Noh, M. Y., Lee, Y. S., Cha, S. J., Seo, S. J., Kim, I., Han, Y. S., & Barillas-Mury, C. (2010). Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes. PloS one, 5(11), [e15410]. https://doi.org/10.1371/journal.pone.0015410