Anxiolytic effects of 5-HT 1A receptors and anxiogenic effects of 5-HT 2C receptors in the amygdala of mice

Qian Li; Tian Luo; Xue Jiang; Jing Wang

doi:10.1016/j.neuropharm.2011.09.002

Anxiolytic effects of 5-HT _1A receptors and anxiogenic effects of 5-HT _2C receptors in the amygdala of mice

Qian Li, Tian Luo, Xue Jiang, Jing Wang

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

The aim of the present study is to test a hypothesis that 5-HT _1A and 5-HT _2C receptors in the amygdala play an important role in the regulation of anxiety behaviors. We examined alterations in anxiety-like behaviors after manipulation of the expression of 5-HT _1A and 5-HT _2C receptors in the amygdala using recombinant adenovirus approaches. Recombinant adenoviruses containing a 5-HT _1A promoter-controlled 5-HT _1A antisense sequence or a 5-HT _2C promoter-controlled 5-HT _2C sense sequence were injected into the amygdala. Elevated plus-maze (EPM) and open field tests were conducted to determine anxiety-like behavior and locomotor activity. Reductions in the expression of 5-HT _1A receptors in the amygdala significantly attenuated the time spent in the open arms of EPM and time spent in the center of an open field. Reduction in the percent of time spent in the open arms of EPM is negatively correlated with the density of 5-HT _1A receptors in the central amygdala. On the other hand, increased expression of 5-HT _2C receptors reduced the time spent in the open arms of EPM and time spent in the center of an open field. The reductions in the time spent and distance traveled in the open arms of EPM were correlated to the density of 5-HT _2C receptors in the basolateral nucleus of amygdala. These data suggest that amygdaloid 5-HT _1A receptors produce anxiolytic and 5-HT _2C receptors produce anxiogenic effects. Together, the present results demonstrate the important role of the amygdaloid 5-HT _1A and 5-HT _2C receptors in the regulation of anxiety-like behaviors. This article is part of a Special Issue entitled 'Anxiety and Depression'.

Original language	English (US)
Pages (from-to)	474-484
Number of pages	11
Journal	Neuropharmacology
Volume	62
Issue number	1
DOIs	https://doi.org/10.1016/j.neuropharm.2011.09.002
State	Published - Jan 2012
Externally published	Yes

Keywords

5-HT receptors
5-HT receptors
Amygdala
Anxiety-like behaviors
Recombinant adenovirus

ASJC Scopus subject areas

Pharmacology
Cellular and Molecular Neuroscience

Access to Document

10.1016/j.neuropharm.2011.09.002

Cite this

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title = "Anxiolytic effects of 5-HT 1A receptors and anxiogenic effects of 5-HT 2C receptors in the amygdala of mice",

abstract = "The aim of the present study is to test a hypothesis that 5-HT 1A and 5-HT 2C receptors in the amygdala play an important role in the regulation of anxiety behaviors. We examined alterations in anxiety-like behaviors after manipulation of the expression of 5-HT 1A and 5-HT 2C receptors in the amygdala using recombinant adenovirus approaches. Recombinant adenoviruses containing a 5-HT 1A promoter-controlled 5-HT 1A antisense sequence or a 5-HT 2C promoter-controlled 5-HT 2C sense sequence were injected into the amygdala. Elevated plus-maze (EPM) and open field tests were conducted to determine anxiety-like behavior and locomotor activity. Reductions in the expression of 5-HT 1A receptors in the amygdala significantly attenuated the time spent in the open arms of EPM and time spent in the center of an open field. Reduction in the percent of time spent in the open arms of EPM is negatively correlated with the density of 5-HT 1A receptors in the central amygdala. On the other hand, increased expression of 5-HT 2C receptors reduced the time spent in the open arms of EPM and time spent in the center of an open field. The reductions in the time spent and distance traveled in the open arms of EPM were correlated to the density of 5-HT 2C receptors in the basolateral nucleus of amygdala. These data suggest that amygdaloid 5-HT 1A receptors produce anxiolytic and 5-HT 2C receptors produce anxiogenic effects. Together, the present results demonstrate the important role of the amygdaloid 5-HT 1A and 5-HT 2C receptors in the regulation of anxiety-like behaviors. This article is part of a Special Issue entitled 'Anxiety and Depression'.",

keywords = "5-HT receptors, 5-HT receptors, Amygdala, Anxiety-like behaviors, Recombinant adenovirus",

author = "Qian Li and Tian Luo and Xue Jiang and Jing Wang",

note = "Funding Information: The authors thank Dr. Bert Vogelstin in The Johns Hopkins oncology center for kindly providing adenoviral vectors and Dr. Tong-Chuan He for his excellent technical advice in the generation of recombinant adenovirus. The authors thank Kate Davis and Yan Liu for their important technical assistance with the experiments. The authors also thank Dr. Nancy A Muma in the University of Kansas, who kindly provided feedback and proof-reading of the manuscript. The studies were supported by USPHS MH72938 (Q.L) and a NARSAD Young Investigator Award to Qian Li. ",

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N1 - Funding Information: The authors thank Dr. Bert Vogelstin in The Johns Hopkins oncology center for kindly providing adenoviral vectors and Dr. Tong-Chuan He for his excellent technical advice in the generation of recombinant adenovirus. The authors thank Kate Davis and Yan Liu for their important technical assistance with the experiments. The authors also thank Dr. Nancy A Muma in the University of Kansas, who kindly provided feedback and proof-reading of the manuscript. The studies were supported by USPHS MH72938 (Q.L) and a NARSAD Young Investigator Award to Qian Li.

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N2 - The aim of the present study is to test a hypothesis that 5-HT 1A and 5-HT 2C receptors in the amygdala play an important role in the regulation of anxiety behaviors. We examined alterations in anxiety-like behaviors after manipulation of the expression of 5-HT 1A and 5-HT 2C receptors in the amygdala using recombinant adenovirus approaches. Recombinant adenoviruses containing a 5-HT 1A promoter-controlled 5-HT 1A antisense sequence or a 5-HT 2C promoter-controlled 5-HT 2C sense sequence were injected into the amygdala. Elevated plus-maze (EPM) and open field tests were conducted to determine anxiety-like behavior and locomotor activity. Reductions in the expression of 5-HT 1A receptors in the amygdala significantly attenuated the time spent in the open arms of EPM and time spent in the center of an open field. Reduction in the percent of time spent in the open arms of EPM is negatively correlated with the density of 5-HT 1A receptors in the central amygdala. On the other hand, increased expression of 5-HT 2C receptors reduced the time spent in the open arms of EPM and time spent in the center of an open field. The reductions in the time spent and distance traveled in the open arms of EPM were correlated to the density of 5-HT 2C receptors in the basolateral nucleus of amygdala. These data suggest that amygdaloid 5-HT 1A receptors produce anxiolytic and 5-HT 2C receptors produce anxiogenic effects. Together, the present results demonstrate the important role of the amygdaloid 5-HT 1A and 5-HT 2C receptors in the regulation of anxiety-like behaviors. This article is part of a Special Issue entitled 'Anxiety and Depression'.

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