Antracene-9-carboxylic acid inhibits renal chloride reabsorption

H. Oberleithner, M. Ritter, F. Lang, William B Guggino

Research output: Contribution to journalArticle

Abstract

From previous studies, it is known that in the diluting segment, Cl--ions are transported from the tubule lumen into the cell together with Na+ and K+ via a furosemide-sensitive cotransport system. This carrier-mediated process, located in the luminal cell membrane, is driven by the steep "downhill" Na+ gradient (directed from lumen to cell) which is maintained by the ouabain-sensitive Na+/K+-pump at the peritubular cell membrane. Cl--ions are accumulated within the cell cytosol and are supposed to leave the cell by a Cl--conductive pathway. The present experiments, performed in diluting segments of the isolated perfused frog kidney, demonstrate the existence of a significant Cl--permeability of the peritubular cell membrane and its complete inhibition by anthracene-9-COOH. The data indicate that Cl--reabsorption can be reduced not only by the inhibition of luminal Cl--entry (i.e. by furosemide) but also by the blockade of the passive Cl--exit step across the peritubular cell membrane. Since complete inhibition of Cl--permeability reduces transepithelial uphill Cl--transport only to half, the data disclose the existance of an additional Cl--pathway at the peritubular cell membrane.

Original languageEnglish (US)
Pages (from-to)172-174
Number of pages3
JournalPflugers Archiv European Journal of Physiology
Volume398
Issue number2
DOIs
StatePublished - Jul 1983

Fingerprint

Cell membranes
Carboxylic Acids
Chlorides
Cell Membrane
Furosemide
Ions
Cell Membrane Permeability
Active Biological Transport
Ouabain
Anura
Cytosol
Permeability
Kidney
Renal Reabsorption
Pumps
Experiments

Keywords

  • Amphibian kidney
  • Anthracene-9-COOH
  • Chloride permeability
  • Diluting segment

ASJC Scopus subject areas

  • Physiology

Cite this

Antracene-9-carboxylic acid inhibits renal chloride reabsorption. / Oberleithner, H.; Ritter, M.; Lang, F.; Guggino, William B.

In: Pflugers Archiv European Journal of Physiology, Vol. 398, No. 2, 07.1983, p. 172-174.

Research output: Contribution to journalArticle

Oberleithner, H. ; Ritter, M. ; Lang, F. ; Guggino, William B. / Antracene-9-carboxylic acid inhibits renal chloride reabsorption. In: Pflugers Archiv European Journal of Physiology. 1983 ; Vol. 398, No. 2. pp. 172-174.
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