Antracene-9-carboxylic acid inhibits renal chloride reabsorption

H. Oberleithner, M. Ritter, F. Lang, W. Guggino

Research output: Contribution to journalArticle

Abstract

From previous studies, it is known that in the diluting segment, Cl--ions are transported from the tubule lumen into the cell together with Na+ and K+ via a furosemide-sensitive cotransport system. This carrier-mediated process, located in the luminal cell membrane, is driven by the steep "downhill" Na+ gradient (directed from lumen to cell) which is maintained by the ouabain-sensitive Na+/K+-pump at the peritubular cell membrane. Cl--ions are accumulated within the cell cytosol and are supposed to leave the cell by a Cl--conductive pathway. The present experiments, performed in diluting segments of the isolated perfused frog kidney, demonstrate the existence of a significant Cl--permeability of the peritubular cell membrane and its complete inhibition by anthracene-9-COOH. The data indicate that Cl--reabsorption can be reduced not only by the inhibition of luminal Cl--entry (i.e. by furosemide) but also by the blockade of the passive Cl--exit step across the peritubular cell membrane. Since complete inhibition of Cl--permeability reduces transepithelial uphill Cl--transport only to half, the data disclose the existance of an additional Cl--pathway at the peritubular cell membrane.

Original languageEnglish (US)
Pages (from-to)172-174
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume398
Issue number2
DOIs
StatePublished - Jul 1 1983

    Fingerprint

Keywords

  • Amphibian kidney
  • Anthracene-9-COOH
  • Chloride permeability
  • Diluting segment

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this