Pretreatment of AT3 rat prostatic carcinoma cells expressing the inhibitor of apoptosis bcl-2 (AT3-bcl-2 cells) with alpha interferon (IFN-α) affected replication of a virulent strain of Sindbis virus (SV) but did not protect against virus-induced cell death. Treatment of cells with IFN-α late during infection affected ongoing SV replication very little. Previous studies have shown that cross-linking of the vital glycoprotein E2 with antibody delays the inhibition of K+ influx by improving the function of Na+K+ATPase and the Na+-K+-2Cl- cotransport system in SV-infected cells (P. Despres, J. W. Griffin, and D. E. Griffin, J. Virol. 69:7006-7014, 1995). In these studies, we have shown that treatment of infected cells with anti-E2 monoclonal antibody also restored the ability of IFN-α to induce antiviral activity in infected cells late during infection. The very low rate of virus release in SV-infected cells treated simultaneously with anti-E2 monoclonal antibody and IFN-α was postulated to be linked to inhibition of virus maturation. Synergistic effects of antibody and IFN-α are likely to be important fur control of SV replication in vivo.
ASJC Scopus subject areas
- Insect Science