Antitumor immunity elicited by 4-1BBL gene transfected Hepa1-6 in vivo

Xiaohong Li, Xiaodong Li, Jianyong Huang, Lixin Wei, Lieping Chen, Mengchao Wu, Yajun Guo

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To study the effects of 4-1BBL on antitumor immunity induced in vivo by murine 4-1BBL gene transfected Hepa1-6. METHODS: Retrovirus vector was used to transfer the 4-1BBL gene into syngeneic murine heptocellular carcinoma cell line Hepa1-6. The products were termed as Hepa1-6/4-1BBL, and then the TCV4-1BBL was obtained by treating them with mitomycin (MMC). Three models (immunological model, early model, and later model) were established to study the antitumor effects of TCV4-1BBL. RESULTS: (1)In immunological models, the syngeneic mice were completely protected by inoculation with TCV4-1BBL, survived free from tumor for a long period (over 100 days). (2)In early models (7 days after inoculation), Hepa1-6 tumor cells showed strong immunogenicity effects and (3) In later models (14 days after inoculation), they had obvious antitumor effects and most of the tumors were disappeared. CONCLUSIONS: The antitumor effect against syngeneic murine hepatocellular carcinoma in vivo is obviously enhanced by treating them with TCV4-1BBL

Original languageEnglish (US)
Pages (from-to)409-412
Number of pages4
JournalZhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Volume10
Issue number6
StatePublished - 2002
Externally publishedYes

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