Antitoxic immunity to cholera in dogs immunized orally with cholera toxin

N. F. Pierce, W. C. Cray, P. F. Engel

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Cholera toxin was evaluated as an oral immunogen against experimental canine cholera. Dogs were immunized orally with 100-μg doses of purified cholera toxin or comparable doses of crude toxin. Both doses caused moderate diarrhea in most nonimmune dogs. Repeated oral doses (12 doses in 54 days) gave marked protection against the diarrheal effect of oral toxin, provoked a vigorous antitoxic response in jejunal mucosa, and gave nearly complete protection against subsequent oral challenge with living virulent Vibrio cholerae. Protection appeared to be due largely to the antitoxic response in intestinal mucosa. The effectiveness of cholera toxin as an oral vaccine contrasts with the previously described ineffectiveness of toxoid given orally. This study provides an example of mucosal immunity due to a nonreplicating vaccine given orally and suggests that cholera toxin may be useful as a component of an oral vaccine for cholera.

Original languageEnglish (US)
Pages (from-to)632-637
Number of pages6
JournalInfection and immunity
Issue number2
StatePublished - 1980

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases


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