Antisense ribosomal protein gene expression specifically disrupts oogenesis in Drosophila melanogaster

S. Qian, S. Hongo, M. Jacobs-Lorena

Research output: Contribution to journalArticlepeer-review

Abstract

To assess the functional importance of ribosomal protein rpA1 gene expression during development of Drosophila melanogaster, we have transformed into the fly's genome an antisense rpA1 gene driven by a heat shock promoter. Antisense rpA1 expression severely disrupted oogenesis and produced a 'small egg' female-sterile phenotype. The severities of these defects were proportional to the level of antisense rpA1 expression. Anti-rpA1 expression did not affect larval or pupal development. Quantitative RNA analysis suggested that high anti-rpA1 expression results in a general decrease of mRNA in the ovary.

Original languageEnglish (US)
Pages (from-to)9601-9605
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number24
DOIs
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • General

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