Antioxidant effect of ethanolic extract of Piper betle Linn. (Paan) on erythrocytes from patients with HbE-beta thalassemia

Phalguni Srimani, Goutam Mandal, Sudipto Ganguly, Piu Saha, Rupashree Sen, Rajib De, Arnab Chatterjee, Maitrayee Bhattacharyya, Utpal Chaudhuri, Susanta K. Bandyopadhyay, Mitali Chatterjee

Research output: Contribution to journalArticle

Abstract

HbE-beta thalassemia is caused by an interaction between HbE and defective b globin gene of thalassemia. Repeated blood transfusions cause an iron overload, triggering an enhanced generation of free radicals. In the present study, the anti-oxidant property of ethanolic extract of the leaves of Piper betle Linn. (PB) was evaluated in the erythrocytes from patients with HbE-beta thalassemia. In patients with HbE-beta thalassemia (n = 30) and age- and sex-matched healthy individuals (n = 30), the baseline level of reactive oxygen species (ROS) and free radical scavenging activity in the erythrocytes was measured by flow cytometry using dihydrodichlorofluorescein diacetate (H2DCFDA), in terms of the geometric mean fluorescence channel (GMFC). The baseline generation of ROS was significantly higher in the erythrocytes from patients with HbE-beta thalassemia, as compared to healthy volunteers, the GMFC being 67.20 ± 4.64 vs. 23.03 ± 1.88 (p<0.001), which was effectively decreased by PB. Similarly, H2O2 (0.5-1.0 mM) induced a higher increase in the GMFC in the erythrocytes from patients with HbE-beta thalassemia, as compared to controls which was effectively reduced by PB. Taken together, PB showed promising anti-oxidant activity against the erythrocytes from patients with HbE-beta thalassemia.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
JournalIndian Journal of Geo-Marine Sciences
Volume46
Issue number3
StatePublished - Jan 1 2009
Externally publishedYes

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Keywords

  • Anti-oxidant
  • Erythrocytes
  • HbE beta thalassemia
  • Piper betle Linn
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Oceanography

Cite this

Srimani, P., Mandal, G., Ganguly, S., Saha, P., Sen, R., De, R., Chatterjee, A., Bhattacharyya, M., Chaudhuri, U., Bandyopadhyay, S. K., & Chatterjee, M. (2009). Antioxidant effect of ethanolic extract of Piper betle Linn. (Paan) on erythrocytes from patients with HbE-beta thalassemia. Indian Journal of Geo-Marine Sciences, 46(3), 241-246.