Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment

Zachary T. Schafer, Alexandra R. Grassian, Loling Song, Zhenyang Jiang, Zachary Gerhart-Hines, Hanna Y. Irie, Sizhen Gao, Pere Puigserver, Joan S. Brugge

Research output: Contribution to journalArticle

Abstract

Normal epithelial cells require matrix attachment for survival, and the ability of tumour cells to survive outside their natural extracellular matrix (ECM) niches is dependent on acquisition of anchorage independence. Although apoptosis is the most rapid mechanism for eliminating cells lacking appropriate ECM attachment, recent reports suggest that non-apoptotic death processes prevent survival when apoptosis is inhibited in matrix-deprived cells. Here we demonstrate that detachment of mammary epithelial cells from ECM causes an ATP deficiency owing to the loss of glucose transport. Overexpression of ERBB2 rescues the ATP deficiency by restoring glucose uptake through stabilization of EGFR and phosphatidylinositol-3-OH kinase (PI(3)K) activation, and this rescue is dependent on glucose-stimulated flux through the antioxidant-generating pentose phosphate pathway. Notably, we found that the ATP deficiency could be rescued by antioxidant treatment without rescue of glucose uptake. This rescue was found to be dependent on stimulation of fatty acid oxidation, which is inhibited by detachment-induced reactive oxygen species (ROS). The significance of these findings was supported by evidence of an increase in ROS in matrix-deprived cells in the luminal space of mammary acini, and the discovery that antioxidants facilitate the survival of these cells and enhance anchorage-independent colony formation. These results show both the importance of matrix attachment in regulating metabolic activity and an unanticipated mechanism for cell survival in altered matrix environments by antioxidant restoration of ATP generation.

Original languageEnglish (US)
Pages (from-to)109-113
Number of pages5
JournalNature
Volume461
Issue number7260
DOIs
StatePublished - Sep 3 2009
Externally publishedYes

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Oncogenes
Antioxidants
Adenosine Triphosphate
Extracellular Matrix
Glucose
Reactive Oxygen Species
Cell Survival
Breast
Epithelial Cells
Apoptosis
Pentose Phosphate Pathway
Phosphatidylinositol 3-Kinases
Fatty Acids
Neoplasms

ASJC Scopus subject areas

  • General

Cite this

Schafer, Z. T., Grassian, A. R., Song, L., Jiang, Z., Gerhart-Hines, Z., Irie, H. Y., ... Brugge, J. S. (2009). Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment. Nature, 461(7260), 109-113. https://doi.org/10.1038/nature08268

Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment. / Schafer, Zachary T.; Grassian, Alexandra R.; Song, Loling; Jiang, Zhenyang; Gerhart-Hines, Zachary; Irie, Hanna Y.; Gao, Sizhen; Puigserver, Pere; Brugge, Joan S.

In: Nature, Vol. 461, No. 7260, 03.09.2009, p. 109-113.

Research output: Contribution to journalArticle

Schafer, ZT, Grassian, AR, Song, L, Jiang, Z, Gerhart-Hines, Z, Irie, HY, Gao, S, Puigserver, P & Brugge, JS 2009, 'Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment', Nature, vol. 461, no. 7260, pp. 109-113. https://doi.org/10.1038/nature08268
Schafer ZT, Grassian AR, Song L, Jiang Z, Gerhart-Hines Z, Irie HY et al. Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment. Nature. 2009 Sep 3;461(7260):109-113. https://doi.org/10.1038/nature08268
Schafer, Zachary T. ; Grassian, Alexandra R. ; Song, Loling ; Jiang, Zhenyang ; Gerhart-Hines, Zachary ; Irie, Hanna Y. ; Gao, Sizhen ; Puigserver, Pere ; Brugge, Joan S. / Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment. In: Nature. 2009 ; Vol. 461, No. 7260. pp. 109-113.
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