TY - JOUR
T1 - Antimycobacterial activity of the antiinflammatory agent diclofenac sodium, and its synergism with streptomycin
AU - Dutta, Noton K.
AU - Dastidar, Sujata G.
AU - Kumar, Asok
AU - Mazumdar, Kaushiki
AU - Ray, Raja
AU - Chakrabarty, Atindra N.
PY - 2004
Y1 - 2004
N2 - Diclofenac sodium, an antiinflammatory agent, exhibited remarkable inhibitory action against both drug sensitive and drug resistant clinical isolates of Mycobacterium tuberculosis, as well as other mycobacteria. This drug was tested in vitro against 45 different strains of mycobacteria, most of which were inhibited by the drug at 10-25 μg/ml concentration. When tested in vivo, diclofenac, injected at 10 μg/g body weight of a Swiss strain of white mice, could significantly protect them when challenged with 50 median lethal dose of M. tuberculosis H37 Rv 102. According to Χ2 test, the in vivo data were highly significant (p≤0.01). Diclofenac was further tested for synergism with the conventional antimycobacterial drug streptomycin against M. smegmatis 798. When compared with their individual effects, synergism was found to be statistically significant (p≤0.05). By the checkerboard assessment procedure, the fractional inhibitory concentration index of this combination was found to be 0.37, confirming synergism.
AB - Diclofenac sodium, an antiinflammatory agent, exhibited remarkable inhibitory action against both drug sensitive and drug resistant clinical isolates of Mycobacterium tuberculosis, as well as other mycobacteria. This drug was tested in vitro against 45 different strains of mycobacteria, most of which were inhibited by the drug at 10-25 μg/ml concentration. When tested in vivo, diclofenac, injected at 10 μg/g body weight of a Swiss strain of white mice, could significantly protect them when challenged with 50 median lethal dose of M. tuberculosis H37 Rv 102. According to Χ2 test, the in vivo data were highly significant (p≤0.01). Diclofenac was further tested for synergism with the conventional antimycobacterial drug streptomycin against M. smegmatis 798. When compared with their individual effects, synergism was found to be statistically significant (p≤0.05). By the checkerboard assessment procedure, the fractional inhibitory concentration index of this combination was found to be 0.37, confirming synergism.
KW - Antiinflammatory drug
KW - Antimycobacterial activity
KW - Diclofenac sodium
KW - Non-antibiotic
KW - Streptomycin
KW - Synergism
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U2 - 10.1590/S1517-83822004000300009
DO - 10.1590/S1517-83822004000300009
M3 - Article
AN - SCOPUS:25144467579
VL - 35
SP - 316
EP - 323
JO - Revista de Microbiologia
JF - Revista de Microbiologia
SN - 1517-8382
IS - 4
ER -