TY - JOUR
T1 - Antimicrobial susceptibility and characterization of outer membrane proteins of Haemophilus ducreyi isolated in Thailand
AU - Taylor, D. N.
AU - Echeverria, P.
AU - Hanchalay, S.
AU - Pitarangsi, C.
AU - Slootmans, L.
AU - Piot, P.
PY - 1985
Y1 - 1985
N2 - One hundred strains of Haemophilus ducreyi isolated in Thailand from patients with chancroid were tested by the agar dilution method against 10 antimicrobial agents and typed by outer membrane protein pattern by using sodium dodecyl sulfate gel electrophoresis. All strains produced beta-lactamase and were resistant to tetracycline, kanamycin, and sulfonamides. Most had a decreased susceptibility to trimethoprim (MIC for 50% of the strains [MIC50], 0.5 μg/ml and chloramphenicol (MIC50, 8 μg/ml). Strains were susceptible to ciprofloxacin (MIC90, 0.001 μg/ml), ceftriaxone (MIC50, 0.0015 μg/ml), erythromycin (MIC50, 0.015 μg/ml), rosoxacin (MIC50, 0.03 μg/ml), and spectinomycin (MIC50, 8 μg/ml). The degree of antimicrobial resistance found in Thailand is higher than that reported for H. ducreyi isolated in other regions. Five different outer membrane protein patterns were found by analyzing proteins in the range of 29 to 61 kilodaltons, but 98% of the Thai strains fell into three patterns which did not differ greatly. Outer membrane protein patterns of Thai strains were also seen in strains from other geographic areas. A new outer membrane protein type was found among nine strains isolated in Singapore.
AB - One hundred strains of Haemophilus ducreyi isolated in Thailand from patients with chancroid were tested by the agar dilution method against 10 antimicrobial agents and typed by outer membrane protein pattern by using sodium dodecyl sulfate gel electrophoresis. All strains produced beta-lactamase and were resistant to tetracycline, kanamycin, and sulfonamides. Most had a decreased susceptibility to trimethoprim (MIC for 50% of the strains [MIC50], 0.5 μg/ml and chloramphenicol (MIC50, 8 μg/ml). Strains were susceptible to ciprofloxacin (MIC90, 0.001 μg/ml), ceftriaxone (MIC50, 0.0015 μg/ml), erythromycin (MIC50, 0.015 μg/ml), rosoxacin (MIC50, 0.03 μg/ml), and spectinomycin (MIC50, 8 μg/ml). The degree of antimicrobial resistance found in Thailand is higher than that reported for H. ducreyi isolated in other regions. Five different outer membrane protein patterns were found by analyzing proteins in the range of 29 to 61 kilodaltons, but 98% of the Thai strains fell into three patterns which did not differ greatly. Outer membrane protein patterns of Thai strains were also seen in strains from other geographic areas. A new outer membrane protein type was found among nine strains isolated in Singapore.
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U2 - 10.1128/jcm.21.3.442-444.1985
DO - 10.1128/jcm.21.3.442-444.1985
M3 - Article
C2 - 3872313
AN - SCOPUS:0021962622
VL - 21
SP - 442
EP - 444
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 3
ER -